HUVECs functions influenced by topographical features on titanium
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1
Peking University School and Hospital of Stomatology, Department of Geriatric Dentistry, China
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2
the Affiliated Stomatology Hospital, College of Medicine, Zhejiang University, Department of Oral and Maxillofacial Surgery, China
In contrast to the intensive researches that focus on the relationships between surface topographical features on titanium (Ti) and its osteogenic activities, less attention has been paid to the relationships between surface topographical features on Ti and its angiogenesis effects, whereas the latter may also play a fundamental role in the process of peri-implant bone healing. The purpose of this study is to reveal the role of different scale structures on the functions of HUVECs (from smooth surface (SM), to nano structured (AE), micron scale structured (SLA) and mico/nano hybrid structured (MS) Ti surfaces). Based on a well established multi-scale structure model [1], we study the cellular behaviors of HUVECs that influenced by different topographical features on Ti, including cell adhesion, proliferation, VEGF production and angiogenesis tube formation ability.
The results showed that the adhesion and proliferation behaviors of HUVECs on Ti surface decreased as the surface structure on titanium evolved (from SM to AE, SLA and MS), as well as the VEGF production and angiogenesis tube formation ability. Redox-sensitive process plays a major role in ECs sensing physical stimulus (known as mechanotransduction) and produces a signal that acts on modifying cell structure, metabolism, and gene expression [2]. Therefore, in order to discuss the underlying mechanism of modulated HUVECs functions by different surface structures on Ti, the reactive oxygen species (ROS) expression of four groups was detected. The results showed that ROS expression of HUVECs on AE-Ti, SLA-Ti and MS-Ti when compared to the SM-Ti. These phenomena suggested that HUVECs displayed a certain degree of dysfunction on AE-Ti, SLA-Ti and MS-Ti caused by the surface topographical feature stimulating, and during which mechanotransduction [2] may play an important role, and ROS was also involved in. We hope that this study will help to gain more insight into the progress of peri-implant bone healing and guide the design of the new implant systems that possess excellent biological activity.
Financial supports by the National Natural Science Foundation of China (81400563) and the National Science-technology Support Plan project of China (2012BAI07B01).
References:
[1] Huang Y, Zha G, Luo Q, Zhang J, Zhang F, Li X, Zhao S, Zhu W, Li X. The construction of hierarchical structure on Ti substrate with superior osteogenic activity and intrinsic antibacterial capability. Sci Rep. 2014, 22(4)：6172.
[2] Chatterjee S, Fisher AB. Mechanotransduction in the endothelium: role of membrane proteins and reactive oxygen species in sensing, transduction, and transmission of the signal with altered blood flow. Antioxid Redox Signal. 2014, 20(6):899-913.
Keywords:
Implant,
Cell response,
stimuli-response,
surface topolography
Conference:
10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016.
Presentation Type:
Poster
Topic:
Biocompatibility of nanobiomaterials
Citation:
Huang
Y,
Liang
N,
Yan
H,
Ma
Q,
Li
X and
Deng
X
(2016). HUVECs functions influenced by topographical features on titanium.
Front. Bioeng. Biotechnol.
Conference Abstract:
10th World Biomaterials Congress.
doi: 10.3389/conf.FBIOE.2016.01.01564
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Received:
27 Mar 2016;
Published Online:
30 Mar 2016.
*
Correspondence:
Dr. Ying Huang, Peking University School and Hospital of Stomatology, Department of Geriatric Dentistry, Beijing, China, Email1
Dr. Naiwen Liang, Peking University School and Hospital of Stomatology, Department of Geriatric Dentistry, Beijing, China, luthienliang2008@hotmail.com