Construction and characterization of protein-encapsulated electrospun fibermats prepared from a silica/poly(γ- glutamate) hybrid
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1
Nagoya Institute of Technology, Graduate School of Engineering, Japan
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2
Imperial College London, Faculty of Engineering, Department of Materials, United Kingdom
Protein-encapsulated fibermats are an attractive platform for protein-based bioactive materials. But the choice of methods is still limited and the previously described methods are not broadly amenable to a wide-range of proteins. In this study, therefore we studied new polymeric material for constructing protein-encapsulated fibermats, in which protein molecules are encapsulated within the nanofibers of fibermats without causing deleterious changes to protein structure or function. We constructed a protein-encapsulated fibermat using the poly(g-glutamate) (PGA)/(3-glycidyloxypropyl)-trimethoxysilane (GPTMS) hybrid as a precursor for electrospinning. Since the PGA/GPTMS hybrid is water soluble, protein molecules can be added to the precursor in an aqueous solution, significantly enhancing protein stability. Polycondensation during electrospinning (in-flight polycondensation) makes the obtained fibermats water-insoluble, which stabilizes the fibermat structure so that it is resistant to degradation in aqueous buffer. The molecular structure of the PGA/GPTMS hybrid gives rise to unique molecular permeability, which alters the selectivity and specificity of biochemical reactions involving the encapsulated enzymes; lower molecular-weight (MW) substrates can permeate the nanofibers, promoting enzyme activity, but higher MW substrates such as inhibitor peptides cannot permeate the nanofibers, suppressing enzyme activity. We present an effective method of encapsulating bioactive molecules while maintaining their structure and function, increasing the versatility of electrospun fibermats for constructing various bioactive materials.
The present work was carried out under the framework of Academic Unit Cooperation Program between Frontier Research Institute for Materials Science, Nagoya Institute of Technology and Department of Materials, Imperial College London.
Keywords:
nanofiber,
protein,
Biofunction
Conference:
10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016.
Presentation Type:
Poster
Topic:
Nano-structured materials for unique functions
Citation:
Mizuno
T,
Obata
A,
Koeda
S,
Shimada
M,
Mizuno
K,
Iguchi
M,
Jones
J and
Kasuga
T
(2016). Construction and characterization of protein-encapsulated electrospun fibermats prepared from a silica/poly(γ- glutamate) hybrid.
Front. Bioeng. Biotechnol.
Conference Abstract:
10th World Biomaterials Congress.
doi: 10.3389/conf.FBIOE.2016.01.01546
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Received:
27 Mar 2016;
Published Online:
30 Mar 2016.
*
Correspondence:
Dr. Toshihisa Mizuno, Nagoya Institute of Technology, Graduate School of Engineering, Nagoya, Japan, Email1
Dr. Akiko Obata, Nagoya Institute of Technology, Graduate School of Engineering, Nagoya, Japan, obata.akiko@nitech.ac.jp
Dr. Makoto Shimada, Nagoya Institute of Technology, Graduate School of Engineering, Nagoya, Japan, m.shimada.853@nitech.jp
Dr. Koji Mizuno, Nagoya Institute of Technology, Graduate School of Engineering, Nagoya, Japan, 24111144@stn.nitech.ac.jp
Dr. Makito Iguchi, Nagoya Institute of Technology, Graduate School of Engineering, Nagoya, Japan, cjh12008@nitech.jp
Dr. Julian Jones, Imperial College London, Faculty of Engineering, Department of Materials, London, United Kingdom, julian.r.jones@imperial.ac.uk
Dr. Toshihiro Kasuga, Nagoya Institute of Technology, Graduate School of Engineering, Nagoya, Japan, kasuga.toshihiro@nitech.ac.jp