Carbon nanotubes embedded in embryoid bodies direct cardiac differentiation
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1
University of Toronto, Institute of Biomaterials and Biomedical Engineering, Canada
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2
Tohoku University, Graduate School of Environmental Studies, Japan
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3
National Institute for Materials Science (NIMS), Japan
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4
Tohoku University, WPI-Advanced Institute for Materials Research, Japan
Carbon nanotubes (CNTs) were embedded in mouse embryoid bodies (EBs) for providing electrical and mechanical cues of the stem cell microenvironment. The CNTs enhanced the stiffness and electrical conductivity of the EBs as measured by atomic force microscopy and current-voltage curves. The EB-CNTs also showed lower proliferation and greater differentiation over time compared with the pristine EBs. Interestingly, the cardiac differentiation of the EB-CNTs was significantly greater than that of the pristine EBs, as confirmed by high-throughput gene analysis and protein expression at days 5 and 10 of culture. Applying electrical stimulation (voltage, 3 V; duration, 10 ms; and frequency, 1 Hz for 2 continuous days) to the EB-CNTs specifically enhanced the cardiac differentiation and beating activity of the EBs. We believe that the proposed approach for cardiac differentiation of EBs will garner great attention in tissue engineering and cell-based therapy applications.
WPI-Advanced Institute for Materials Research
Keywords:
Cell Differentiation,
stem cell,
nanotube
Conference:
10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016.
Presentation Type:
Poster
Topic:
Nano-structured materials for unique functions
Citation:
Ahadian
S,
Yamada
S,
Estili
M,
Liang
X,
Nakajima
K,
Shiku
H and
Matsue
T
(2016). Carbon nanotubes embedded in embryoid bodies direct cardiac differentiation.
Front. Bioeng. Biotechnol.
Conference Abstract:
10th World Biomaterials Congress.
doi: 10.3389/conf.FBIOE.2016.01.00801
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Received:
27 Mar 2016;
Published Online:
30 Mar 2016.