Cell-type specific circuit analysis using a digital brain atlas which integrates anatomical and physiological data
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1
Karolinska Institutet, Sweden
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2
Max Planck Institute for Medical Research, Germany
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3
The Rockefeller University, United States
In BAC (Bacterial artificial chromosome) transgenic mice we investigated the anatomical and functional properties of two populations of layer 5 pyramids in somatosensory and visual cortex. The two populations were selectively labelled by EGFP expressed under the control of cell-type specific promoters. The genotypic profile discriminated between thick-tufted corticopontine and slender-tufted corticostriatal layer 5 pyramids. The projection target specificity of the two populations was determined by retrograde tracer injections and visualization of EGFP-labelled axons in the target area. Using the fluorescent labelling we could target our investigation to a specific cell-type in layer 5 and perform (i) electrophysiological recordings to investigate cell-type specific intrinsic membrane properties and connectivity (ii) a quantification of the dendrite morphology and (iii) an analysis of the spatial distribution profiles of somata from the two populations and (iv) an investigation of the overlap of thalamocortical axons and dendrites from the two populations of corticofugal layer 5 pyramids.
We show that targeting cell types using BAC-transgenic mice could be a valuable approach to generate atlases consisting of both detailed anatomical data, including both cell-type specific output and input, and physiological data relevant for functional circuit analysis.
Conference:
Neuroinformatics 2008, Stockholm, Sweden, 7 Sep - 9 Sep, 2008.
Presentation Type:
Poster Presentation
Topic:
General Neuroinformatics
Citation:
Krieger
P,
Sakmann
B,
Groh
A,
Meyer
H and
Heintz
N
(2008). Cell-type specific circuit analysis using a digital brain atlas which integrates anatomical and physiological data.
Front. Neuroinform.
Conference Abstract:
Neuroinformatics 2008.
doi: 10.3389/conf.neuro.11.2008.01.007
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Received:
28 Jul 2008;
Published Online:
28 Jul 2008.
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Correspondence:
Patrik Krieger, Karolinska Institutet, Stockholm, Sweden, patrik.krieger@rub.de