The Electrical Activity of CA1 Areas in Acutely 7-Nitroindazole Treated Rats in Ischemia-Reperfusion
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1
Istanbul University, Türkiye
Nitric oxide (NO), produced by nitric oxide synthase (NOS), is an important endogenous vasodilatator. In this study we investigated the effects of 7-nitrindazole (7-NI), a NOS inhibitor, to improved our understanding of NO physiology by investigating electrical activity on hippocampal CA1 regions in rats subjected to cerebral ischemia-reperfusion (I/R). Male Wistar albino rats, anaesthetized with thiopental (50 mg/kg i.p.), were implanted with bilateral electrodes in CA1 areas in stereotaxic apparatus. One week after operation, continuous EEG recordings were taken under anesthesia for five minutes. Then animals were randomly divided into two groups as saline treated control group (n=6) and 50 mg/kg 7-NI treated 7-NI group (n=6). One day after injection, bilateral carotid arteries of all animals were clamped. Thirty minutes after occlusion, clamps were re-opened and EEG recordings were taken in following ten minutes. Continuous EEG recordings were divided into 2-second epochs and analyzed by averaged frequency spectra (0.5-50 Hz), then two tailed paired Student’s t-test was applied. In control group animals, I/R induced the increase in amplitudes of low frequency bands and decrease in amplitudes of high frequency bands in CA1 regions significantly compared to basal values. In 7-NI group animals, the amplitudes of only high frequency bands were significantly decreased in CA1 areas. This study showed that acute treatment with 50 mg/kg 7-NI, before the rats were subjected to I/R, has protective effects on electrical activity on hippocampal CA1 areas in low and high (30 Hz and up) frequency bands.
Conference:
10th International Conference on Cognitive Neuroscience, Bodrum, Türkiye, 1 Sep - 5 Sep, 2008.
Presentation Type:
Poster Presentation
Topic:
Animal Models of Cognition
Citation:
Zengin
A,
Tekin
D,
Kayacilar
C,
Yildiz
R,
Nurten
A and
Kara
I
(2008). The Electrical Activity of CA1 Areas in Acutely 7-Nitroindazole Treated Rats in Ischemia-Reperfusion.
Conference Abstract:
10th International Conference on Cognitive Neuroscience.
doi: 10.3389/conf.neuro.09.2009.01.083
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Received:
02 Dec 2008;
Published Online:
02 Dec 2008.
*
Correspondence:
Asli Zengin, Istanbul University, Istanbul, Türkiye, aslizenginn@yahoo.com