NMDA Receptor Mediated Dendritic Spikes in CA1 Stratum Radiatum Interneurons
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1
Institute of Experimental Medicine, Hungarian Academy of Sciences, Hungary
Although the anatomical properties of glutamatergic synapses on dendrites of hippocampal interneurons are well characterized, the source and pharmacological property of calcium transients as a consequence of the glutamatergic neurotransmission remain to be clarified. In order to study the pharmacology of the calcium transients evoked by excitatory synaptic transmission in the dendrites of CA1 hippocampal interneurons, two-photon imaging was used in combination with focal synaptic stimulation and rapid drug application. Our data revealed that local administration of AP5, an NMDA receptor antagonist could significantly attenuate the amplitude of both locally evoked calcium transients and EPSPs in the distal dendritic segments of interneurons. The role of NMDA receptors as the main source of calcium influx was further supported by whole-cell recordings performed in Mg-free ACSF that resulted in robust elevation of synaptic calcium influx and amplitude of EPSPs. Moreover, increasing stimulation for excitatory inputs evoked local dendritic spikes (dSpikes) that could be described with larger peak amplitude of the calcium transients paired with a larger amplitude and slower decay of EPSPs. The dSpikes could not be detected when AP5 was administered locally. These results indicate that in the dendrites of CA1 interneurons NMDA receptors might serve as the main source of glutamatergic neurotransmission evoked calcium influx. Furthermore, focal synaptic stimulation is able to evoke dSpikes, which are also NMDA receptor dependent. The dSpikes described in our experiments may have a key role in dendritic signal integration.
Conference:
12th Meeting of the Hungarian Neuroscience Society, Budapest, Hungary, 22 Jan - 24 Jan, 2009.
Presentation Type:
Poster Presentation
Topic:
Research on the cerebral cortex and related structures
Citation:
Turi
G,
Kaszas
A,
Katona
G,
Chiovini
B,
Szilveszter
VE and
Rozsa
B
(2009). NMDA Receptor Mediated Dendritic Spikes in CA1 Stratum Radiatum Interneurons.
Front. Syst. Neurosci.
Conference Abstract:
12th Meeting of the Hungarian Neuroscience Society.
doi: 10.3389/conf.neuro.01.2009.04.224
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Received:
09 Mar 2009;
Published Online:
09 Mar 2009.
*
Correspondence:
Gergely Turi, Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest, Hungary, turig@koki.hu