Laser scanning microscope for scanning along 3D trajectories with 5 ms time resolution
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1
Institute of Experimental Medicine Hungarian Academy of Sciences, Hungary
We developed a new scanning strategy and a new 3D two-photon microscope for dendritic imaging with 5 ms time resolution. We use our multiple line scan technique (Differential distribution of NCX1 contributes to spine-dendrite compartmentalization in CA1 pyramidal cells (2007) Proc. Natl. Acad. Sci. USA, 10.1073) with the combination of two z-scanning methods. This combination results in movement typical for a roller coaster. Our technique provides 20 µm scanning range along axis z when it is used with 6 ms repetition rate. The spatial resolution (namely the mean half width of the point spread function) is better than 500 nm. We optimized our detectors for scattered photons; therefore, our system provides an excellent signal to noise ratio when compared to commercially available systems. Using our method, we measured the mechanism of the initiation and spread of dendritic regenerative activity (dendritic spikes) induced by focal electric stimulation. Our technique revealed a new form of dendritic spikes and synaptic integration in hippocampal CA1 stratum radiatum interneurons.
Conference:
12th Meeting of the Hungarian Neuroscience Society, Budapest, Hungary, 22 Jan - 24 Jan, 2009.
Presentation Type:
Poster Presentation
Topic:
Research on the cerebral cortex and related structures
Citation:
Katona
G,
Chiovini
B,
Vizi
SE and
Rozsa
B
(2009). Laser scanning microscope for scanning along 3D trajectories with 5 ms time resolution.
Front. Syst. Neurosci.
Conference Abstract:
12th Meeting of the Hungarian Neuroscience Society.
doi: 10.3389/conf.neuro.01.2009.04.204
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Received:
06 Mar 2009;
Published Online:
06 Mar 2009.
*
Correspondence:
Gergely Katona, Institute of Experimental Medicine Hungarian Academy of Sciences, Budapest, Hungary, katonag@koki.hu