Presence of pituitary adenylate cyclase activating polypeptide in human serum and milk
Rita
Borzsei1,
Laszlo
Mark2,
Andrea
Tamas3,
Terez
Bagoly1,
Csaba
Bay4,
Katalin
Csanaky3,
Eszter
Banki3,
Peter
Kiss3,
Alexandra
Vaczy2,
Gabriella
Horváth3,
Jozsef
Nemeth5,
Edit
Szauer3,
Zsuzsanna
Helyes1 and
Dóra
Reglődi3*
-
1
Departments of Pharmacology and Pharmacotherapeutics, University of Pecs, Hungary
-
2
Departments of Biochemistry and Medical Chemistry, University of Pecs, Hungary
-
3
Departments of Anatomy, University of Pecs, Hungary
-
4
Departments of Anatomy , University of Pecs, Hungary
-
5
Department of Pharmacology and Pharmacotherapeutics, University of Debrecen, Hungary
Pituitary adenylate cyclase activating polypeptide (PACAP) is a pleiotropic and multifunctional neuropeptide distributed throughout the body. Since little is known about its distribution in humans, our aim was to examine PACAP-38 in human plasma and milk. The presence of PACAP-38 was determined by mass spectrometry in plasma samples from healthy male and female volunteers (age: 20-40), in plasma and milk samples from lactating women (age: 20-35). PACAP concentration was measured with a specific and sensitive radioimmunoassay. Our results revealed that PACAP-38 is present in human plasma, its concentration is relatively stable and it is not significantly altered by gender, age, food intake or hormonal cycle in females. However, PACAP-38 plasma levels increased in lactating women having 1-6 month-old babies. Moreover, this study is the first which provides evidence for the presence of PACAP-38 in milk with levels 5-20-fold greater in the milk whey than in the respective plasma samples. We found PACAP-38 in human plasma and its increase during the first 6 months of the lactation period. A prominent, almost a 10-fold concentration of this peptide was detected in human milk. Important actions of milk-derived PACAP-38 can be suggested such as mammary gland proliferation, nutrient transfer and regulation of growth/differentiation of neonate tissues. The novelty of the present interesting descriptive data provide a basis for further investigations onthe mechanism of PACAP-38 accumulation in human milk and its functional significance.
Support: OTKA K72592, K73044, F67830, K75965, 78480, Bolyai Fellowship
Conference:
12th Meeting of the Hungarian Neuroscience Society, Budapest, Hungary, 22 Jan - 24 Jan, 2009.
Presentation Type:
Poster Presentation
Topic:
Homeostatic regulatory mechanisms
Citation:
Borzsei
R,
Mark
L,
Tamas
A,
Bagoly
T,
Bay
C,
Csanaky
K,
Banki
E,
Kiss
P,
Vaczy
A,
Horváth
G,
Nemeth
J,
Szauer
E,
Helyes
Z and
Reglődi
D
(2009). Presence of pituitary adenylate cyclase activating polypeptide in human serum and milk.
Front. Syst. Neurosci.
Conference Abstract:
12th Meeting of the Hungarian Neuroscience Society.
doi: 10.3389/conf.neuro.01.2009.04.116
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Received:
04 Mar 2009;
Published Online:
04 Mar 2009.
*
Correspondence:
Dóra Reglődi, Departments of Anatomy, University of Pecs, Pecs, Hungary, dora.reglodi@aok.pte.hu