Effect of alachlor on the metabolome of wild type and GmGSTU4 overexpressing transgenic tobacco plants (Nicotiana tabacum L.)
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1
Aristotle University of Thessaloniki, Department of Genetics and Plant Breeding, Greece
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2
Agricultural University of Athens, Department of Agricultural Biotechnology, Greece
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3
Institute of Agrobiotechnology, CERTH, Greece
Plant glutathione transferases (GSTs) comprises an enzyme superfamily tightly connected with crop herbicide tolerance. The overexpression of GmGSTU4 isoenzyme from soybean in transgenic tobacco plants has been previously shown to confer increased tolerance to the chloroacetanilide herbicide alachlor. Here we studied the effect of alachlor treatment (3mg/L) on the metabolome of in vitro grown wild type and GmGSTU4 overexpressing tobacco plants of cv. Basmas. Metabolites were identified using gas chromatography/mass spectrometry (GC/MS). Alachlor treatment had a significant effect in the level of several metabolites in wild type plants compared to control. Particularly a significant decrease was observed in the relative concentration of pyruvate, succinate , maltose, melibiose and tryptophan. In contrast, mannitol, hydroquinone and proline were significantly increased. Comparing the metabolome of wild type and GmGSTU4 overexpressing plants grown in 3mg/L alachlor, several metabolites were identified as differentially altered. Transgenic plants exhibited significantly increased levels of acetyl-serine, a precursor in the biosynthetic pathway of glutathione, a major determinant of alachlor detoxification. An increase was also observed in tryptophan, proline, maltose, melibiose and hydroquinone. Metabolites significantly decreased compared to wild type plants were glucose, fructose and manittol. Taken together these results suggest that the increased tolerance to alachlor of GmGSTU4 overexpressing tobacco plants is an outcome of sustained metabolic homoeostasis and enhanced detoxification potential that leads to the continuation of growth in the presence of the herbicide.
Keywords:
glutathione transferases,
tobacco plants,
transgenic,
alachlor
Conference:
8th Southeast European Congress on Xenobiotic Metabolism and Toxicity - XEMET 2010, Thessaloniki, Greece, 1 Oct - 5 Oct, 2010.
Presentation Type:
Poster
Topic:
Genomics - Proteomics - Metabolomics
Citation:
Kissoudis
C,
Axarli
A,
Labrou
NE,
Madesis
P,
Tsaftaris
A and
Nianiou-Obeidat
I
(2010). Effect of alachlor on the metabolome of wild type and GmGSTU4 overexpressing transgenic tobacco plants (Nicotiana tabacum L.).
Front. Pharmacol.
Conference Abstract:
8th Southeast European Congress on Xenobiotic Metabolism and Toxicity - XEMET 2010.
doi: 10.3389/conf.fphar.2010.60.00144
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Received:
28 Oct 2010;
Published Online:
04 Nov 2010.
*
Correspondence:
Dr. Irini Nianiou-Obeidat, Aristotle University of Thessaloniki, Department of Genetics and Plant Breeding, Thessaloniki, GR-54124, Greece, nianiou@agro.auth.gr