Regulation of dendritogenesis by ZBP1 depends on its phosphorylation at Ser181
-
1
International Institute of Molecular and Cell Biology, Poland
Zipcode Binding Protein 1 (ZBP1) is one of proteins involved in local translation, a mechanism present in polarized cells, enabling rapid, localized protein synthesis in response to extracellular stimuli. It was previously shown that in neurons, processes coordinated by ZBP1 are indispensable for proper axonal growth cone and spine formation. We recently showed that developing neurons with overexpressed or knockdown ZBP1 cannot obtain proper morphology. We also proved that phosphorylation of ZBP1 by Src kinase is needed for proper dendritic branching [1]. Subsequently, we asked a question about other regulators of ZBP1 during dendritogenesis. Now, we demonstrate that ZBP1 is effectively phosphorylated in vitro by mTOR kinase. We took advantage of recently published data regarding potential mTOR-dependent phosphorylation sites in ZBP1 i.e. Ser181 [2] and examined role of this phosphorylation in (i) dendritic arborization and (ii) cellular distribution of ZBP1. To address these questions, we constructed non-phosphorable (S181A) and phosphomimicking (S181E) mutants of ZBP1 fused to GFP. We observed that S181E but not S181A reversed morphological deficits caused by ZBP1 knockdown. Another observation was that distribution along the dendrites of non-phosphorable mutant was more even than distribution of wild type ZBP1, which is denser at the dendritic branching points. Thus, we concluded that Ser181 phosphorylation is involved in ZBP1 functions during dendritic growth.
Acknowledgements
This research has been supported by National Science Center (NCN) grant 2011/01/N/NZ3/05405, ICGEB grant CRP/ POL11-02 and Mazovia grant.
References
[1] M. Perycz, A.S. Urbanska, P.S. Krawczyk, K. Parobczak, J. Jaworski, J Neurosci, 2011, 31(14), 5271-85.
[2] N. Dai, J. Rapley, M. Angel, M.F. Yanik, M.D. Blower, J. Avruch, mTOR phosphorylates IMP2 to promote IGF2 mRNA translation by internal ribosomal entry. Genes Dev, 2011, 25(11):1159-72.
Keywords:
ZBP1,
mTOR,
local translation,
Dendritogenesis,
IMP1
Conference:
4th NAMASEN Training Workshop - Dendrites 2014, Heraklion, Greece, 1 Jul - 4 Jul, 2014.
Presentation Type:
Poster presentation
Topic:
RNA trafficking and local protein synthesis
Citation:
Urbanska
AS and
Jaworski
J
(2014). Regulation of dendritogenesis by ZBP1 depends on its phosphorylation at Ser181.
Front. Syst. Neurosci.
Conference Abstract:
4th NAMASEN Training Workshop - Dendrites 2014.
doi: 10.3389/conf.fnsys.2014.05.00038
Copyright:
The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers.
They are made available through the Frontiers publishing platform as a service to conference organizers and presenters.
The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated.
Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed.
For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions.
Received:
11 Apr 2014;
Published Online:
12 Jun 2014.
*
Correspondence:
Ms. Anna S Urbanska, International Institute of Molecular and Cell Biology, Warsaw, 02-109, Poland, annaurbanska@iimcb.gov.pl