Human iPSC derived neurons recorded by MEA technology: A powerful tool for functional assessment of in vitro neuropharmacology and neurotoxicity
-
1
Axiogenesis, Germany
-
2
Axiogenesis, United States
In vitro pharmacology profiling of new chemical entities during early phases of drug discovery has recently become an essential tool to predict clinical adverse effects. While for cardiac safety testing high technology platforms are available, specific in vitro neurotoxic panels are not, and in-vivo models are used instead. However, correlations between animal and human data are often weak; in addition,animal studies are expensive, ethically questionable and require large amounts of chemical compounds.
In order to directly address human targets, we developed a 48-well platform based on the usage of human induced pluripotent stem-cell derived neurons. Compound related effects on the spontaneous activity of the Peri.4U neurons were assessed by performing field potential recordings using multiwell microelectrode array (MEA)- technology (Maestro System, Axion, Atlanta).
When cultivated on the MEA chips, spontaneous activity of these neurons was recorded in 100% of the wells after a cultivation period of 3-4 days. Recordings with clear detectable burst-like activity indicating the presence and establishment of a functional neuronal network were chosen for testing neuropharmacological and neurotoxic compounds. Agonists and blockers of common neurotransmitter receptors as well as reference compounds with known neurotoxic potency were analyzed for their effect on neuronal behavior, thereby demonstrating the potency of this in vitro system for reliable detection and quantification of neuropharmacological and neurotoxic compound actions.
Keywords:
Neuropharmacology,
Neurotoxicity,
MEA,
in vitro,
IPSC-derived neurons
Conference:
MEA Meeting 2016 |
10th International Meeting on Substrate-Integrated Electrode Arrays, Reutlingen, Germany, 28 Jun - 1 Jul, 2016.
Presentation Type:
Poster Presentation
Topic:
MEA Meeting 2016
Citation:
Hess
D,
Luerman
G and
Bohlen
H
(2016). Human iPSC derived neurons recorded by MEA technology: A powerful tool for functional assessment of in vitro neuropharmacology and neurotoxicity.
Front. Neurosci.
Conference Abstract:
MEA Meeting 2016 |
10th International Meeting on Substrate-Integrated Electrode Arrays.
doi: 10.3389/conf.fnins.2016.93.00032
Copyright:
The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers.
They are made available through the Frontiers publishing platform as a service to conference organizers and presenters.
The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated.
Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed.
For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions.
Received:
22 Jun 2016;
Published Online:
24 Jun 2016.
*
Correspondence:
Dr. Dietmar Hess, Axiogenesis, Köln, Germany, dietmar.hess@axiogenesis.com