Recording Single-Unit Data From Acute Cortical Slices On A High-Density (300 electrodes/mm2) 512-Channel Micro-Needle Array
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1
University of Strathclyde, Institute of Photonics, Department of Physics, United Kingdom
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2
AGH University of Science and Technology, Institute of Physics, Poland
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3
University of California, Santa Cruz, SCIPP, United States
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4
Indiana University, Biocomplexity Institute, United States
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5
University of California, Santa Cruz, SCIPP, United States
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6
University of Strathclyde, Institute of Photonics, Department of Physics, United Kingdom
Acute brain slices are an important model for the electrophysiological study of neural connectivity in vitro. A technological approach to bypassing the intrinsically damaged surface layer of these freshly cut slices, to record simultaneously from many connected neurons in the in tact interior volume of the slice, is to use of arrays of penetrating microelectrodes. The current state-of-the-art in devices of this nature work very well to record from neurons in acute (and in vivo) neural preparations but have widely spaced needles and as a result, limited spatial resolution. Addressing this limitation, we present a novel, high-density penetrating micro-needle electrode array and its performance in experiments with acute cortical slices from wild type rats. The developed micro-needle array has the unprecedented combination of: high electrode count (512); large area coverage (1mm x 2mm); high electrode density (60 μm pitch); variable needle heights (up to 300 μm); and small electrode diameters (down to 5μm). As an excellent first result, the array was shown to record from >50 acute cortical neurons simultaneously, in a single preparation, and with a signal to noise of ~10:1.
Keywords:
Electrodes,
Cortex,
Micro-needle,
Acute slice
Conference:
MEA Meeting 2016 |
10th International Meeting on Substrate-Integrated Electrode Arrays, Reutlingen, Germany, 28 Jun - 1 Jul, 2016.
Presentation Type:
oral
Topic:
MEA Meeting 2016
Citation:
Young
D,
Hottowy
P,
Dabrowski
W,
Sher
A,
Beggs
J,
Litke
A,
Mathieson
K and
Gunning
DE
(2016). Recording Single-Unit Data From Acute Cortical Slices On A High-Density (300 electrodes/mm2) 512-Channel Micro-Needle Array.
Front. Neurosci.
Conference Abstract:
MEA Meeting 2016 |
10th International Meeting on Substrate-Integrated Electrode Arrays.
doi: 10.3389/conf.fnins.2016.93.00022
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Received:
22 Jun 2016;
Published Online:
24 Jun 2016.
*
Correspondence:
Dr. Deborah E Gunning, University of Strathclyde, Institute of Photonics, Department of Physics, Glasgow, United Kingdom, deborah.gunning@strath.ac.uk