Dual-mode-operation Microelectrode Array for Extracellular Action Potential Recording
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1
ETH Zurich, Department of Biosystems Science and Engineering, Switzerland
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2
Riken, Quantitative Biology Center, Japan
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3
ETH Zurich, Department of Biosystems Science and Engineering, Switzerland
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4
ETH Zurich, Department of Biosystems Science and Engineering, Switzerland
Motivation
A variety of readout schemes has been demonstrated for CMOS-based high-density microelectrode arrays (HDMEA), each featuring advantages and disadvantages [1]. Active-pixel sensor (APS) arrays, for example, enable simultaneous recording from thousands of electrodes but with sub-optimal SNR, while switch-matrix (SM) devices enable recording at best possible SNR, whereas only a subset of electrodes can be recorded from simultaneously. Here, a dual-mode operation MEA (DM-MEA) is presented with the aim to leverage the advantages of APS and SM arrays by realizing a combined architecture. We developed a specific reconstruction algorithm to process the raw data acquired by the APS readout. For many experiments, especially studies of neuronal network activity, the DM-MEA is an enabling technology, since it provides high throughput and high SNR at the same time. An example would be the study of the propagation of action potentials on full axonal arbors.
Material and Methods
The array is composed of 96×96 pixels with an electrode density of 3050 mm-2. Pt electrodes were fabricated through post-processing, using a previously published shifted-electrode design [2]. For APS, there are 9216 readout channels with a frame rate of 6.5 kHz, while the SM readout includes 112 channels at a sampling rate of 20 kHz.
Results
The DM-MEA has been fabricated and tested [3]. Measured noise levels were 11.1 µVrms for APS and 1.6 µVrms for SM mode (spike sorting potential band, 300 Hz – 5 kHz) at a power consumption of 3.3 µW (APS) and 38.1 µW (SM) per channel. Experiments have been done with cerebellar acute slices and cultured neuron cells using SM and APS readout simultaneously.
Conclusion
The concept of a DM-MEA is presented. Owing to its flexibility in choosing readout modes and stimulation routings, the DM-MEA is a versatile tool that is applicable to various neuroscience studies with a broad range of requirements.
References
[1]. M. E. J. Obien, K. Deligkaris, T. Bullmann, D. J. Bakkum, and U. Frey, “Revealing neuronal function through microelectrode array recordings,” Front. Neurosci., vol. 8, no. 423, Jan. 2015.
[2]. U. Frey, U. Egert, F. Heer, S. Hafizovic, and A. Hierlemann, “Microelectronic System for High-Resolution Mapping of Extracellular Electric Fields Applied to Brain Slices,” Biosensors and Bioelectronics, vol. 24, no. 7, pp. 2191-2198, 2009.
[3]. X. Yuan, S. Kim, J. Juyon, M. D’Urbino, T. Bullmann, Y. Chen, A. Stettler, A. Hierlemann, U. Frey, “A Microelectrode Array with 8,640 Electrodes Enabling Simultaneous Full-frame Readout at 6.5 kfps and 112-Channel Switch-Matrix Readout at 20 kS/s”, VLSI Circuits (VLSIC), 2016 Symposium on, June. 2016.
Acknowledgements
Financial support through the ERC Advanced Grant 267351 “NeuroCMOS” is acknowledged.
Conference:
MEA Meeting 2016 |
10th International Meeting on Substrate-Integrated Electrode Arrays, Reutlingen, Germany, 28 Jun - 1 Jul, 2016.
Presentation Type:
Poster Presentation
Topic:
MEA Meeting 2016
Citation:
Yuan
X,
Kim
S,
D’Urbino
M,
Juyon
J,
J. Obien
ME,
Bullmann
T,
Chen
Y,
Stettler
A,
Hierlemann
A and
Frey
U
(2016). Dual-mode-operation Microelectrode Array for Extracellular Action Potential Recording.
Front. Neurosci.
Conference Abstract:
MEA Meeting 2016 |
10th International Meeting on Substrate-Integrated Electrode Arrays.
doi: 10.3389/conf.fnins.2016.93.00014
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Received:
22 Jun 2016;
Published Online:
24 Jun 2016.
*
Correspondence:
Dr. Xinyue Yuan, ETH Zurich, Department of Biosystems Science and Engineering, Basel, Switzerland, xinyue.yuan@bsse.ethz.ch