Multi-Functional CMOS-HDMEA Integrating 59'760 Electrodes, 2'048 Electrophysiology Readouts, Stimulation Units, Impedance and Neurotransmitter Measurement Units
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1
ETH Zurich, Department of Biosystems Science and Engineering, Switzerland
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2
ETH Zurich, Department of Biosystems Science and Engineering, Switzerland
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3
ETH Zurich, Department of Biosystems Science and Engineering, Switzerland
Motivation
In order to study the electrophysiological properties of electrogenic cells, state-of-the art HDMEAs include, at most, voltage readout and voltage/current stimulation units [1]-[4]. While voltage readout provides insight into cell information processing, it is not sufficient to reveal all the electro-chemical phenomena taking place in and around the cells and at the cell-electrode interface. In an attempt to better understand these complex phenomena, we developed a novel multi-functional HDMEA, which integrates several different types of readout and stimulation units in a single system.
Material and Methods
The novel MEA features 2’048 action-potential and 32 local-field-potential readout channels, 32 current recording units, 32 impedance and 28 neurotransmitter measurement units, and 16 dual-mode voltage/current stimulation units. The system is built around an array of 59’760 electrodes at 13.5µm pitch (5487 electrodes/µm2) in an area of 4.48x2.43mm2. The array is based on the switch-matrix structure [3], [4]. Its electrodes can be simultaneously and flexibly connected to the various readout/stimulation units for simultaneous, multi-functional investigation of a cell/tissue preparation. Array reconfiguration takes up to 4.5ms.
Results
The voltage recording units yield an input-referred noise level as low as 2.4µVrms in the action potential frequency band, which is equal to our earlier designs [3], [4], and significantly less than the state-of-the-art [1], [2], [5].
Conclusion
The presented HDMEA is the first of its kind that includes many different readout and stimulation units on a single substrate for parallel investigation of different cellular parameters. It also features the largest sensing area to date, with an electrode pitch as small as 13.5µm and excellent noise performance.
Acknowledgements
The authors thank M. Ballini, P. Livi, D. Bakkum and U. Frey for help. Financial support through ERC Advanced Grant 267351 “NeuroCMOS” and individual support for A. Shadmani through FP7-MTN “EngCaBra” (Contract 264417) is acknowledged.
Keywords:
neurotransmitter,
Integrated,
CMOS,
impedance,
In-vitro,
Multi-functional,
high-density
Conference:
MEA Meeting 2016 |
10th International Meeting on Substrate-Integrated Electrode Arrays, Reutlingen, Germany, 28 Jun - 1 Jul, 2016.
Presentation Type:
oral
Topic:
MEA Meeting 2016
Citation:
Dragas
J,
Viswam
V,
Shadmani
A,
Chen
Y,
Stettler
A,
Radivojevic
M,
Müller
J and
Hierlemann
A
(2016). Multi-Functional CMOS-HDMEA Integrating 59'760 Electrodes, 2'048 Electrophysiology Readouts, Stimulation Units, Impedance and Neurotransmitter Measurement Units.
Front. Neurosci.
Conference Abstract:
MEA Meeting 2016 |
10th International Meeting on Substrate-Integrated Electrode Arrays.
doi: 10.3389/conf.fnins.2016.93.00002
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Received:
22 Jun 2016;
Published Online:
24 Jun 2016.
*
Correspondence:
Dr. Jelena Dragas, ETH Zurich, Department of Biosystems Science and Engineering, Basel, Switzerland, jelena.r.dragas@gmail.com