Effects of intraamygdaloid microinjections of RFRP-1 on liquid food intake of rats
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1
Pécs University Medical School, Institute of Physiology, Hungary
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2
Pécs University Medical School, Neurophysiology Research Group of the HAS, Hungary
RFRP-1 is a member of the RF-amide peptide family. Since some members of the RFamide family (NPFF, NPVF, NPAF) are associated with satiety in rodents, we hypothesized that RFRP-1 may also affect satiety. It is well known, that the amygdala plays an important role in the regulation of food intake and body weight. RFRP-1-positive nerve cells were detected in the rat hypothalamus and RFRP-1 immunreactive fibers were identified in the central amygdaloid nucleus (CeA) by immunohistochemical analyses. RFRP analogues bind with relatively high affinity to the NPFF1 and NPFF2 receptors. RFRP-1 has potent activity for NPFF-1 that is expressed in the CeA. To evaluate the role of RFRP-1 in appetite regulation, rats were microinjected by different doses of RFRP-1 and their food intake were quantified over a 60 min period. Liquid food intake of male wistar rats was measured after bilateral intraamygdalar administration of RFRP-1 (25, 50, 100 ng /side, RFRP-1 dissolved in 0.15 M sterile NaCl/0.4 µl, respectively). The 50 ng dose of RFRP-1 microinjections resulted in significant decrease in food intake. The 25 and 100 ng had no effect. Action of 50 ng (37.8 pmol) RFRP-1 was eliminated by 20ng (41.4 pmol) NPFF-R antagonist (RF-9) pretreatment. Our results are the first reporting that RFRP-1 injected to CeA resulted in a decrease of liquid food consumption. This is a receptor-linked effect because it was eliminated by a NPFF- R selective antagonist.
Acknowledgements
Supported by the NKTH-OTKA K68431 and by the HAS.
Keywords:
Behaviour and cognition,
Neuroscience
Conference:
13th Conference of the Hungarian Neuroscience Society (MITT), Budapest, Hungary, 20 Jan - 22 Jan, 2011.
Presentation Type:
Abstract
Topic:
Behaviour and cognition
Citation:
Kovács
A,
Tóth
K,
László
K,
Gálosi
R,
Ollmann
T and
Lénárd
L
(2011). Effects of intraamygdaloid microinjections of RFRP-1 on liquid food intake of rats.
Front. Neurosci.
Conference Abstract:
13th Conference of the Hungarian Neuroscience Society (MITT).
doi: 10.3389/conf.fnins.2011.84.00164
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Received:
03 Mar 2011;
Published Online:
23 Mar 2011.
*
Correspondence:
Dr. L. Lénárd, Pécs University Medical School, Institute of Physiology, Pécs, Hungary, Laszlo.Lenard@aok.pte.hu