Effects of L-aspartate on single neurons in striatal slice preparation from chicken brain
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1
Semmelweis University, Dept. of Anatomy, Histology and Embryology, Hungary
There is accumulating evidence for a transmitter role of L-aspartate (L-Asp) in various brain regions. Recent studies from our laboratory have indicated that L-Asp is present in excitatory synapses of the striatum/nucl. accumbens of domestic chicks where it is co-released with L-glutamate (L-Glu) from axon terminals. Here we provide data on the postsynaptic effects of L-Asp alongside with L-Glu in striatal slices from chicken (1- to 5-day-old) using visually guided patch-clamp technique. Bath application L-Asp and L-Glu produced similar dose-dependent inward currents and an increase in spontaneous synaptic activity with a threshold concentration around 200 μM in all of the recorded striatal neurons. The amplitude of the inward currents were not substantially modified when the slices were perfused with TTX-containing (1 μM) Krebs solution to block Na spikes and synaptic transmission suggesting a predominant postsynaptic effect of both excitatory amino acids. In the presence of TTX both the NMDA receptor antagonist D-AP5 (25-100 μM) and the AMPA/kainate receptor antagonist CNQX (10-20 μM) reduced and the co-application of these two antagonists abolished the postsynaptic effects of L-Asp and L-Glu in a reversible manner. The data are the first to demonstrate that L-Asp can induce postsynaptic effects on the chicken striatal neurons. These effects are mediated by both NMDA and non-NMDA type ionotropic glutamate receptors and are similar to those evoked by L-Glu. OTKA 73219
Keywords:
Molecular and cellular neurobiology,
Neuroscience
Conference:
13th Conference of the Hungarian Neuroscience Society (MITT), Budapest, Hungary, 20 Jan - 22 Jan, 2011.
Presentation Type:
Abstract
Topic:
Molecular and cellular neurobiology
Citation:
Balázs
D,
Csillag
A and
Gerber
G
(2011). Effects of L-aspartate on single neurons in striatal slice preparation from chicken brain.
Front. Neurosci.
Conference Abstract:
13th Conference of the Hungarian Neuroscience Society (MITT).
doi: 10.3389/conf.fnins.2011.84.00087
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Received:
03 Mar 2011;
Published Online:
23 Mar 2011.
*
Correspondence:
Dr. G. Gerber, Semmelweis University, Dept. of Anatomy, Histology and Embryology, Budapest, Hungary, gerber@ana.sote.hu