Convergence of parvalbumin and type 1 cannabinoid receptor positive perisomatic inputs onto hippocampal pyramidal cells
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1
Hungarian Academy of Sciences, Dept. of Cellular and Network Neurobiology, Institute of Experimental Medicine, Hungary
The soma and proximal dendrites of cortical pyramidal cells are innervated by two functionally distinct types of perisomatic interneurons: the parvalbumin (PV)- and the cholecystokinin-containing, type 1 cannabinoid receptor (CB1) positive basket cells. Here we estimated the proportion of PV- and CB1-positive basket cell inputs in mice by reconstructing parts of hippocampal CA1 pyramidal cell somata and the contacting boutons from serial electron microscopic sections using a new double staining method that gives distinguishable signal at the electron microscopic level. All but 1% of the somatic boutons were labeled by one of the markers. The number of PV- and CB1-positive boutons/soma was ~35 and ~23, respectively. Next, we have measured some of the ultrastructural parameters of 3D reconstructed immunogold-labeled PV- and CB1 positive boutons. The volume occupied by mitochondria was significantly larger in PV-positive boutons. Both types formed more than one synapses often on the same soma or an additional soma or a dendrite. PV-positive boutons possessed small, macular shaped active zones. Synapses of CB1-positive boutons formed a complex structure with multiple small and large irregular shaped active zones. The total synaptic areas/bouton was significantly larger in the case of CB1-positive boutons. Our results show that the physiological differences of the two basket cell types are mirrored by morphological diversity of their boutons.
Acknowledgements
Support: NKTH-OTKA CNK 77793, NIH DA11322.
Keywords:
Molecular and cellular neurobiology,
Neuroscience
Conference:
13th Conference of the Hungarian Neuroscience Society (MITT), Budapest, Hungary, 20 Jan - 22 Jan, 2011.
Presentation Type:
Abstract
Topic:
Molecular and cellular neurobiology
Citation:
Takács
VT,
Freund
TF and
Gulyás
AI
(2011). Convergence of parvalbumin and type 1 cannabinoid receptor positive perisomatic inputs onto hippocampal pyramidal cells.
Front. Neurosci.
Conference Abstract:
13th Conference of the Hungarian Neuroscience Society (MITT).
doi: 10.3389/conf.fnins.2011.84.00051
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Received:
03 Mar 2011;
Published Online:
23 Mar 2011.
*
Correspondence:
Dr. V. T Takács, Hungarian Academy of Sciences, Dept. of Cellular and Network Neurobiology, Institute of Experimental Medicine, Budapest, Hungary, viragt@koki.hu