GLP-1 fibers of brainstem origin activate dorsomedial hypothalamic neurons in refed rats
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1
Semmelweis University and the Hungarian Academy of Sciences, Department of Anatomy, Histology and Embryology, Hungary
Previously, a cell group expressing c-fos in response to refeeding of fasted rats was identified in the ventral subdivision of the hypothalamic dorsomedial nucleus (DMH). Here, we report that Fos is not induced in these cells when food consumption is limited during refeeding suggesting that satiation is an important factor for the activation. Glucagon-like peptide-1 (GLP-1)- and prolactin-releasing peptide (PrRP)-containing nerve terminals topographycally overlap with the area where the activated neurons distribute in the DMH. Ascending fibers of GLP-1 neurons in the nucleus of the solitary tract and PrRP neurons in the lower medulla oblongata were followed in sagittal brain sections as they project towards the DMH. Transections of these fibers at pontine as well as caudal hypothalamic levels resulted in a dramatic decrease in the density of PrRP- and GLP-1 fibers and fiber terminals in the ipsilateral DMH meanwhile a marked decrease was observed in the number of Fos-expressing DMH neurons, in response to refeeding. Using a combination of immunocytochemistry and in situ hybridization histochemistry, we demonstrated the presence of GLP-1 but not PrRP receptors in Fos-expressing neurons of the DMH. These findings suggest that during refeeding, GLP-1-containing solitary-hypothalamic projections contribute to the activation of DMH neurons via GLP-1 receptors possibly by conveying information on satiety.
Acknowledgements
This study was supported by OTKA CK 80180 research grant.
Keywords:
Molecular and cellular neurobiology,
Neuroscience
Conference:
13th Conference of the Hungarian Neuroscience Society (MITT), Budapest, Hungary, 20 Jan - 22 Jan, 2011.
Presentation Type:
Abstract
Topic:
Molecular and cellular neurobiology
Citation:
Renner
É,
Puskás
N,
Dobolyi
Á and
Palkovits
M
(2011). GLP-1 fibers of brainstem origin activate dorsomedial hypothalamic neurons in refed rats.
Front. Neurosci.
Conference Abstract:
13th Conference of the Hungarian Neuroscience Society (MITT).
doi: 10.3389/conf.fnins.2011.84.00023
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Received:
03 Mar 2011;
Published Online:
23 Mar 2011.
*
Correspondence:
Dr. M. Palkovits, Semmelweis University and the Hungarian Academy of Sciences, Department of Anatomy, Histology and Embryology, Budapest, Hungary, palkovits@ana.sote.hu