DECREASED DENSITY OF SOMATOSTATIN-CONTAINING INTERNEURONS IN ADULT RAT BRAIN AFTER PERINATAL PHENCYCLIDINE TREATMENT
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1
School of Medicine, University of Belgrade, Institute of Histology and Embryology, Russia
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2
Center for Molecular Neurobiology Hamburg, Germany
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3
University of Belgrade, Institute of Medical and Clinical Biochemistry,School of Medicine, Russia
GABAergic interneurons perform crucial roles in cortical development and function. Different subclasses of GABA cortical neurons can be distinguished by their content of neuropeptides such as somatostatin (SST) positive cells. SST neurons have been reported to be affected in the prefrontal cortex (PFC) of subjects with schizophrenia. Perinatal phencyclidine (PCP) administration to rats has been established as an animal model of schizophrenia. Our previous studies have demonstrated decreased density of parvalbumin- and reelin-positive interneurons in cortex and hippocampus of the rats perinatally treated with PCP. The aim of this study was to determine effect of perinatal PCP treatment on density of SST containing interneurons in adult rat brain in different forebrain regions.
Animals were treated on 2nd, 6th, 9th and 12th postnatal (PN) day, with either PCP (10 mg/kg) or vehicle (0.9% saline) subcutaneously. Cell densities were estimated on PN70 using the optical dissector principle on a fluorescence microscope equipped with Neurolucida software.
After perinatal PCP administration density of somatostatin containing interneurons was reduced in dentate gyrus (GD) (-50%) and CA3 (-46%) subregions of hippocampus, while there was no significant change in CA1 subregion. In cingulate, motor and retrosplenial cortex there was no significant change in density of these interneurons. These findings indicate that perinatal blockade of NMDA receptor signaling induces a region-specific disruption in development of SST-containing GABA-ergic neurons. This result is especially interesting considering that SST-containing, dendritic targeting neurons appear to be necessary for filtering out distracting stimuli during working memory processing which is impaired in schizophrenia patients.
Conference:
EMBO workshop: Gaba Signalling and Brain Networks , Amsterdam, Netherlands, 30 Jun - 2 Jul, 2010.
Presentation Type:
Poster Presentation
Topic:
Posters
Citation:
Bumbaširevic
V,
Jakovcevski
I,
Petronijevic
ND and
Radonjic
NV
(2010). DECREASED DENSITY OF SOMATOSTATIN-CONTAINING INTERNEURONS IN ADULT RAT BRAIN AFTER PERINATAL PHENCYCLIDINE TREATMENT.
Conference Abstract:
EMBO workshop: Gaba Signalling and Brain Networks .
doi: 10.3389/conf.fnins.2010.15.00019
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Received:
24 Jun 2010;
Published Online:
24 Jun 2010.
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Correspondence:
Nevena V Radonjic, University of Belgrade, Institute of Medical and Clinical Biochemistry,School of Medicine, Belgrade, Russia, radonjin@upstate.edu