Effects of carotenoids on skin inflammation in in vivo mice models
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1
University of Pecs, Department of Pharmacology, Hungary
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2
University of Pecs, Department of Pharmacognosy, Hungary
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3
University of Pecs, Department of Biochemistry and Medical Chemistry, Hungary
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4
Cyclolab Ltd., Hungary
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5
University of Pecs, Department of Pharmacotechnology, Hungary
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6
Hungarian Academy of Science, Chemical Research Center, Hungary
Pro-inflammatory neuropeptides released from capsaicin-sensitive sensory nerve endings in response to the activation of Transient Receptor Potential ion channels, such as Ankyrin repeat 1 (TRPA1) and Vanilloid 1 (TRPV1), induce neurogenic inflammation in the innervated area. Lipid rafts surrounding these receptors regulate their function. Carotenoids are naturally occurring tetraterpenoids, their anti-inflammatory effects are well established, but the mechanism of action is unknown. Since they incorporate into membranes, they might influence the activation of TRP channels and consequently, neurogenic inflammatory processes. Therefore, the aim of this study was to investigate the effects of three carotenoids with different structures (β-carotene, lutein and lycopene) on neurogenic inflammation of the mouse ear induced by topical application of the TRPV1 agonist capsaicin (2,5%) or the TRPA1 agonist mustard oil (5%).
Ear diameter was measured with micrometry and swelling was expressed as percentage. Histological examination and measurement of myeloperoxidase (MPO) activity correlating with the number of accumulated granulocytes were also performed from the ears 6 h after inflammation induction.
Lutein and β-carotene pretreatment (3x100 ug/kg s.c. during 24 h) significantly reduced mustard oil-induced ear oedema throughout the whole 6 h period and also granulocyte accumulation as shown by the histology and MPO activity data. In contrast lycopene had no effect on these inflammatory changes. None of the examined carotenoids altered capsaicin-evoked swelling and granulocyte influx.
These results indicate that lutein containing hydroxyl groups on the α- and β-ionon rings inhibits the activation of TRPA1 receptors and the consequent neurogenic inflammatory responses. Modulation of lipid rafts around the TRPA1 channel may be involved in this action.
Funds: OTKA K 60121 and K 76176.
Conference:
IBRO International Workshop 2010, Pécs, Hungary, 21 Jan - 23 Jan, 2010.
Presentation Type:
Poster Presentation
Topic:
Sensory and motor systems
Citation:
Kemény
A,
Horváth
G,
Molnár
P,
Deli
J,
Szente
L,
Bozó
T,
Pál
S,
Dévay
A,
Simonyi
M,
Szolcsányi
J and
Helyes
Z
(2010). Effects of carotenoids on skin inflammation in in vivo mice models.
Front. Neurosci.
Conference Abstract:
IBRO International Workshop 2010.
doi: 10.3389/conf.fnins.2010.10.00229
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Received:
04 May 2010;
Published Online:
04 May 2010.
*
Correspondence:
Agnes Kemény, University of Pecs, Department of Pharmacology, Pecs, Hungary, kemenyagnes@index.hu