Distribution of Hypophysiotropic Thyrotropin-Releasing Hormone (TRH)-Synthesizing Neurons in the Hypothalamic Paraventricular Nucleus of the Mouse
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1
Institute of Experimental Medicine Hungarian Academy of Sciences, Department of Endocrine Neurobiology, Hungary
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2
Tufts-Medical Center, Tupper Research Institute and Department of Medicine, Division of Endocrinology, Diabetes, and Metabolism, United States
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3
Brigham and Women's Hospital, Thyroid Section, Division of Endocrinology, Diabetes and Hypertension, United States
Hypophysiotropic thyrotropin-releasing hormone (TRH) neurons, the central regulators of the hypothalamus-pituitary-thyroid axis, are located in the hypothalamic paraventricular nucleus (PVN) in a partly overlapping distribution with non-hypophysiotropic TRH neurons. The distribution of hypophysiotropic TRH neurons in the rat PVN is well understood, but the localization of these neurons is unknown in mice. To determine the distribution and phenotype of hypophysiotropic TRH neurons in mice, double-labeling experiments were performed on sections of intact mice, and mice treated intraperitonially with the retrograde tracer Fluoro-Gold. TRH neurons were located in all subnuclei of the PVN except the periventricular parvocellular subdivision. Hypophysiotropic TRH neurons were observed only at the mid level of the PVN, primarily in the magnocellular division, and in the adjacent region of the medial parvocellular subdivision. In the magnocellular division, TRH-neurons were intermingled with oxytocin and vasopressin neurons, but based on their size, the TRH neurons were parvocellular and did not contain magnocellular neuropeptides. Co-localization of TRH and CART were observed only in areas where hypophysiotropic TRH neurons were located. In accordance with the morphological observations, hypothyroidism increased TRH mRNA content of neurons only at the mid portion of the PVN.
These data demonstrate that the distribution of hypophysiotropic TRH neurons in mice is vastly different from the pattern in rats, with a dominant occurrence of these neurosecretory cells in the magnocellular division and adjacent regions of the medial parvocellular subdivision of the PVN.
Conference:
IBRO International Workshop 2010, Pécs, Hungary, 21 Jan - 23 Jan, 2010.
Presentation Type:
Poster Presentation
Topic:
Homeostatic and neuroendocrine systems
Citation:
Kádár
A,
Sánchez
E,
Wittmann
G,
Singru
PS,
Füzesi
T,
Marsili
A,
Larsen
RP,
Liposits
Z,
Lechan
RM and
Fekete
C
(2010). Distribution of Hypophysiotropic Thyrotropin-Releasing Hormone (TRH)-Synthesizing Neurons in the Hypothalamic Paraventricular Nucleus of the Mouse.
Front. Neurosci.
Conference Abstract:
IBRO International Workshop 2010.
doi: 10.3389/conf.fnins.2010.10.00095
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Received:
22 Apr 2010;
Published Online:
22 Apr 2010.
*
Correspondence:
Andrea Kádár, Institute of Experimental Medicine Hungarian Academy of Sciences, Department of Endocrine Neurobiology, Budapest, Hungary, kadara@koki.hu