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Deformable coregistration of multimodal imaging data of gray short-tailed opossum brain

Piotr Majka1*, Natalia Chlodzinska1, Tomasz Banasik2, Ruzanna L. Djavadian1, Władysław P. Węglarz2, Krzysztof Turlejski1 and Daniel K. Wójcik1
  • 1 Nencki Institute of Experimental Biology PAS, Poland
  • 2 H. Niewodniczański Institute of Nuclear Physics PAS, Poland

Gray short-tailed opossum (Monodelphis domestica) becomes increasingly popular laboratory animal, particularly useful in developmental and comparative neurobiology. Despite the numerous studies on the opossum brain anatomy (e.g. [1, 2]), no comprehensive and consistent neuroanatomical reference in the form of a brain atlas is yet available.

In our previous studies ([3], http://goo.gl/Epsoe) we proposed a template of the single Monodelphis opossum's brain comprising four imaging modalities: (1) T1/T2* magnetic resonance image acquired post mortem with Bruker BioSpec 9.4T imaging system with the isotropic resolution of 50um; (2) Images of the front of the tissue block acquired during cryosectioning and two series of slices stained with (3) Nissl method and for (4) myelinated fibers. Images of the stained slices were reconstructed into a consistent volume using rigid transformations and further improved with iterative deformable reconstruction process. Nevertheless, registration between modalities is still performed only with the affine transformations which significantly limits accuracy of the coregistration and poses difficulties during delineation of the brain structures using multiple imaging modalities.

To overcome this issue a deformable multimodal coregistration will be carried out using symmetric diffeomorphic mappings [4]. In the cases when the intensity based registration will not be able to capture anatomical similarities, label-guided mapping will be employed. Corresponding anatomical features will be manually highlighted on the individual modalities to enforce the registration. The improved mapping will facilitate the ongoing process of delineating individual brain structures and the complete template will enhance usefulness of the Monodelphis opossum as a convenient model in neurobiological research.

Figure 1
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Acknowledgements

The project is partly supported by an infrastructural grant from the Polish Ministry of Regional Development POIG.02.03.00-00-003/09.

References

[1] Olkowicz, S., Turlejski, K., Bartkowska, K., Wielkopolska, E., and Djavadian, R. L. (2008). Thalamic nuclei in the opossum Monodelphis domestica. Journal of chemical neuroanatomy, 36(2), 85–97.

[2] Kahn, D. M., and Krubitzer, L. (2002). Retinofugal projections in the short-tailed opossum (Monodelphis domestica). The Journal of comparative neurology, 447(2), 114–27.

[3] Majka, P., Chlodzinska, N., Banasik, T., Djavadian, R. L., Węglarz, W. P., Turlejski, K. and Wójcik, D. K. (2012). Integration of multimodal neuroanatomical data of gray short-tailed opossum. Neuroinformatics 2012 Congress.

[4] Avants, B. B., Tustison, N. J., Song, G., Cook, P. a, Klein, A., and Gee, J. C. (2011). A reproducible evaluation of ANTs similarity metric performance in brain image registration. NeuroImage, 54(3), 2033–44.

Keywords: atlasing, MRI, Magnetic Resonance Imaging, opossum, Histological Techniques, multimodal integration, Multimodal Imaging, Stereotaxic Techniques

Conference: Imaging the brain at different scales: How to integrate multi-scale structural information?, Antwerp, Belgium, 2 Sep - 6 Sep, 2013.

Presentation Type: Poster presentation

Topic: Integrating multi-scale images in standardized atlases

Citation: Majka P, Chlodzinska N, Banasik T, Djavadian R, Węglarz W, Turlejski K and Wójcik D (2013). Deformable coregistration of multimodal imaging data of gray short-tailed opossum brain. Front. Neuroinform. Conference Abstract: Imaging the brain at different scales: How to integrate multi-scale structural information?. doi: 10.3389/conf.fninf.2013.10.00026

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Received: 29 Jul 2013; Published Online: 31 Aug 2013.

* Correspondence: Mr. Piotr Majka, Nencki Institute of Experimental Biology PAS, Warsaw, Poland, p.majka@nencki.edu.pl