Astrocytic structural changes correlate with overall running activities in the globus pallidus
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1
Nara Medical University, Department of Anatomy & Neuroscience, Faculty of Medicine, Japan
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2
Kanazawa University, Department of Functional Anatomy, Graduate School of Medical Sciences, Japan
Astrocytes actively modulate neurotransmission at tripartite synapses consisted of their fine processes and neuronal synapses. Morphological changes of astrocytes are primarily attributed to such processes. During lineage-tracing studies of cells expressing Olig2, we found that Olig2-lineage mature astrocytes preferentially cluster in the globus pallidus that exerts pivotal functions in the indirect pathway of the basal ganglionic circuit. Taking advantage of membrane-targeted GAP43-EGFP, which can visualize the morphology of Olig2-lineage cells in detail, we investigated how running influenced Olig2-lineage astrocyte morphology in the globus pallidus at both the light and electron microscopic levels. Runner group mice were allowed free access to a running wheel for three weeks, and Runner-Rest group mice took an enforced three-week rest after the three-week running period. GFP immunofluorescence analyses and immunoelectron microscopy revealed that astrocytic fine processes elaborated complex arborization in the Runner mice, and reverted to simple morphology in the Runner-Rest group, which is comparable to that in the Control group. The Olig2-lineage astrocytes expressed the GABA transporter GAT-3, suggesting that they participated in inhibitory GABA transmission in the globus pallidus. The fine processes of the Olig2-lineage astrocytes underwent plastic changes that correlated with overall running activities, suggesting that they actively modulate motor functions.
Keywords:
Astrocytes,
Globus Pallidus,
Motor function,
in vivo,
plastic changes
Conference:
14th Meeting of the Asian-Pacific Society for Neurochemistry, Kuala Lumpur, Malaysia, 27 Aug - 30 Aug, 2016.
Presentation Type:
Poster Presentation Session
Topic:
14th Meeting of the Asian-Pacific Society for Neurochemistry
Citation:
Tatsumi
K,
Okuda
H,
Takemura
SM,
Tanaka
T,
Ishonishi
A and
Wanaka
A
(2016). Astrocytic structural changes correlate with overall running activities in the globus pallidus.
Conference Abstract:
14th Meeting of the Asian-Pacific Society for Neurochemistry.
doi: 10.3389/conf.fncel.2016.36.00201
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Received:
04 Aug 2016;
Published Online:
11 Aug 2016.
*
Correspondence:
Prof. Kouko Tatsumi, Nara Medical University, Department of Anatomy & Neuroscience, Faculty of Medicine, Kashihara, Nara, Japan, radha815@naramed-u.ac.jp