The role of neural-differentiated bone marrow stem cells in the re-myelination of regenerated axon
Khairunnisa
Ramli1,
Ifasha
Gasim2,
Amir
Ahmad2,
Ohnmar
Htwe2,
Law
Zhe Kang3,
Azmi
Baharudin2,
Mohd Hisam
Muhamad Ariffin2,
Hazla
Mohammad Haflah2,
Tan
Geok Chin4,
Amaramalar
Selvi Naicker2,
Shariful
Hassan5,
Ruszymah
Hj Idrus1, 6,
Shalimar
Abdullah2 and
Min Hwei
Ng1*
-
1
Universiti Kebangsaan Malaysia Medical Centre, Tissue Engineering Centre, Malaysia
-
2
Universiti Kebangsaan Malaysia Medical Centre, Department of Orthopedic and Traumatology, Malaysia
-
3
Universiti Kebangsaan Malaysia Medical Centre, Department of Medicine, Malaysia
-
4
Universiti Kebangsaan Malaysia Medical Centre, Department of Pathology, Malaysia
-
5
Universiti Putra Malaysia, Department of Medicine, Faculty of Medicine and Health Science, Malaysia
-
6
Universiti Kebangsaan Malaysia, Department of Physiology, Malaysia
Schwann cells (SC) is ideal for use in cell-based therapy for peripheral nerve injuries and demyelination disease. However its procurement is challenging, which includes the requirement of biopsy from healthy nerve followed by the culturing and expanding in vitro to get sufficient cell numbers. Bone marrow-derived MSCs, known with the capability to differentiate into SC-like cells may be a promising alternative source of SC for application in autologous cell therapy. In the present study, we investigated the survival of transplanted human neural-differentiated bone marrow-derived stem cells transduced with green fluorescent protein (NDMSC-GFP) seeded in muscle-stuffed vein and its incorporation in re-myelinated axons after 3 months of implantation. At passage 2, human mesenchymal stem cells from human bone marrow sample were transduced using pCignal Lenti-TRE-Reporter gene bearing green fluorescent protein (GFP) promoter and were cultured until passage 3, followed by neural differentiation process using neural-induction media. Prepared human muscle-stuffed vein seeded with NDMSC-GFP was used to bridge a 15mm of sciatic nerve defect in male athymic rats (n=2). At the end of 12 weeks, the nerve grafts were harvested and analysed histologically and immunohistochemically using S100B and NF200, a specific marker for SC and axon respectively. At 12 weeks after transplantation, GFP-positive tissue sections were observed with morphology similar to native sciatic nerve. By immunohistochemistry, presence of nerve regeneration was confirmed with S100B and NF200 expression. This is evidence of the survival and incorporation of cells in nerve regeneration in vivo. The addition of human neural differentiated cells in conduits may have facilitated the myelination of the regenerated axons.
Keywords:
Bone Marrow,
stem cell,
Axon,
remyelination,
neural-differentiation
Conference:
14th Meeting of the Asian-Pacific Society for Neurochemistry, Kuala Lumpur, Malaysia, 27 Aug - 30 Aug, 2016.
Presentation Type:
Poster Presentation Session
Topic:
14th Meeting of the Asian-Pacific Society for Neurochemistry
Citation:
Ramli
K,
Gasim
I,
Ahmad
A,
Htwe
O,
Zhe Kang
L,
Baharudin
A,
Muhamad Ariffin
M,
Mohammad Haflah
H,
Geok Chin
T,
Selvi Naicker
A,
Hassan
S,
Hj Idrus
R,
Abdullah
S and
Ng
M
(2016). The role of neural-differentiated bone marrow stem cells in the re-myelination of regenerated axon.
Conference Abstract:
14th Meeting of the Asian-Pacific Society for Neurochemistry.
doi: 10.3389/conf.fncel.2016.36.00167
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Received:
04 Aug 2016;
Published Online:
11 Aug 2016.
*
Correspondence:
Dr. Min Hwei Ng, Universiti Kebangsaan Malaysia Medical Centre, Tissue Engineering Centre, Cheras, Kuala Lumpur, Malaysia, angelaster3@gmail.com