Spontaneous differentiation of human full-term amniotic fluid mesenchymal stem cells into post mitotic neurons
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1
Universiti Putra Malaysia, Department of Biomedical Sciences, Malaysia
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2
Britannia Women and Children Specialist Centre, Britannia Women and Children Specialist Centre, Malaysia
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3
Universiti Putra Malaysia, Department of Obstetrics and Gynaecology, Malaysia
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4
Universiti Putra Malaysia, Genetics and Regenerative Medicine Research Centre, Malaysia
Amniotic fluid (AF) is known to contain a few types of stem cells including mesenchymal stem cells (MSCs). MSCs are multipotent stem cells that can differentiate into various cell types and possess immunosuppressive property, hence they serve as one of the best stem cells for regenerative medicine. The aim of this study was to isolate MSCs from full-term human AF using xeno-and serum-free medium; however, the study took a new twist. AF was obtained during caesarean section. The AF cells were cultured in MesenCult™-XF medium, a standardized, xeno- and serum-free medium for the culture of human MSCs. A monolayer spindle shaped fibroblast-like cells were observed attached to the flask. During expansion at passage two, these cells started forming a neuron-like morphology seen during in vitro neural differentiation process. The cells were confirmed positive for class III ß-tubulin; however, they were negative for MAP-2 through immunocytochemistry analysis. Immunophenotyping analysis revealed that more than 90% of the cells population expressed the ß-tubulin protein as well as MSCs markers (CD105, CD73, CD90, CD44 and GD2); however, negative for MAP-2, a marker for mature neurons. These results indicate that the cells exhibited the post-mitotic neuronal cell identity while maintaining the MSCs properties. This study demonstrated that AF-MSCs able to spontaneously differentiate into neural lineage without chemical induction, suggesting its potential therapeutic usage in clinical applications especially in neurodegenerative diseases.
Keywords:
Stem Cells,
in vitro,
neural lineage,
neural differentiation,
Human amniotic fluid
Conference:
14th Meeting of the Asian-Pacific Society for Neurochemistry, Kuala Lumpur, Malaysia, 27 Aug - 30 Aug, 2016.
Presentation Type:
Poster Presentation Session
Topic:
14th Meeting of the Asian-Pacific Society for Neurochemistry
Citation:
Mohd Rawi
RI,
Tan
BC,
Rejali
Z,
Nordin
N and
Thilakavathy
K
(2016). Spontaneous differentiation of human full-term amniotic fluid mesenchymal stem cells into post mitotic neurons.
Conference Abstract:
14th Meeting of the Asian-Pacific Society for Neurochemistry.
doi: 10.3389/conf.fncel.2016.36.00147
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Received:
04 Aug 2016;
Published Online:
11 Aug 2016.
*
Correspondence:
Prof. Karuppiah Thilakavathy, Universiti Putra Malaysia, Department of Biomedical Sciences, UPM Serdang, Selangor, Malaysia, thilathy@upm.edu.my