How to build a complex dendrite arbors? Novel roles of myosin in dendrite branching
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1
RIKEN Brain Science Institute, Laboratory for Genetic Control of Neuronal Architecture, Japan
Neurons require highly branched dendritic arbor morphologies to wire together neural networks and define the individual computational properties of the cell. The mechanisms of how dendritic arbor architectures are established remain unknown. Importantly these mechanisms are often disrupted in neurological disorders and may be utilized during neuronal regeneration. Utilizing non-invasive, in vivo time-lapse microscopy of dendrite arbor morphogenesis in Drosophila pupa, we observed a sequence of distinct phases in the metamorphic dendrite re-outgrowth processes. Each phase has a characteristic set of highly dynamic processes, and developmental transitions between these phases are carefully regulated. Using live imaging-based screening approach, we identified groups of signalling molecules and cytoskeletal modulators that control the dynamics of the processes during each stage and the transitions between phases. The early scaffold building of the dendrite arbor is established through a transient but mobile, dendritic growth cone structures. Using in vivo labeling of actin and microtubule cytoskeletons, we found that stereotypic dendritic branching events were prefigured by the creation of specific cytoskeletal tracks. From our screen, we identified that the atypical myosin, Myosin VI is a key molecule in the sequential building of a highly branched dendrite arbor structure. Myosin VI lays the foundations of dendrite growth cone structures to create the complex dendrite arbor.
Keywords:
Drosophila,
neural networks,
Neuronal regeneration,
dendrite arborization,
Myosin VI
Conference:
14th Meeting of the Asian-Pacific Society for Neurochemistry, Kuala Lumpur, Malaysia, 27 Aug - 30 Aug, 2016.
Presentation Type:
YIC03: Young Investigator Colloquium 3
Topic:
14th Meeting of the Asian-Pacific Society for Neurochemistry
Citation:
Yoong
L and
Moore
A
(2016). How to build a complex dendrite arbors? Novel roles of myosin in dendrite branching.
Conference Abstract:
14th Meeting of the Asian-Pacific Society for Neurochemistry.
doi: 10.3389/conf.fncel.2016.36.00064
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Received:
04 Aug 2016;
Published Online:
11 Aug 2016.
*
Correspondence:
Dr. Li-Foong Yoong, RIKEN Brain Science Institute, Laboratory for Genetic Control of Neuronal Architecture, Wako-shi, Saitama, Japan, yoong_lifoong@brain.riken.jp