Gene expression changes in the rat frontal cortex caused by sex steroid hormones
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1
Florey Institute of Neuroscience and Mental Health, Biological Psychiatry and Mental Health Division, Australia
Growing evidence supports the notion that sex steroids, particularly oestrogen, play an important role in psychiatric disorders. The impact of oestrogen on gene expression in the frontal cortex, a region particularly affected in psychiatric disorders, is unknown. This study aimed to identify alterations in gene expression caused by sex steroid hormone removal and oestrogen treatment. Adult female Sprague-Dawley rats were either intact (sham-operated), ovariectomised, or ovariectomised and treated with 17b-oestradiol for 4 weeks. Frontal cortical RNA was extracted and hybridised to an Agilent SurePrint G3 Rat Gene Expression microarray. Using JMP Genomics, expression data was analysed to identify genes with different expression profiles. These genes were imported into Ingenuity Pathway Analysis to identify pathways affected by ovariectomy and/or oestrogen treatment. Ovariectomy significantly altered the expression profiles of 145 genes, while oestrogen treatment altered the expression profiles of 332 genes. While there was some overlap in altered expression profiles between the ovariectomised animals and those receiving oestrogen, these results highlight that removal of sex steroids by ovariectomy substantially differs from oestrogen treatment. These data suggest that the effects of oestrogen on gene expression will have profound and wide-ranging effects in the frontal cortex, supporting the concept that sex steroids play a role on modulating pathways associated with schizophrenia.
Keywords:
Gene Expression,
Schizophrenia,
Steroids,
neuropsychiatric disorders,
Oestrogen
Conference:
14th Meeting of the Asian-Pacific Society for Neurochemistry, Kuala Lumpur, Malaysia, 27 Aug - 30 Aug, 2016.
Presentation Type:
YIC02: Young Investigator Colloquium 2
Topic:
14th Meeting of the Asian-Pacific Society for Neurochemistry
Citation:
Gogos
A,
Udawela
M,
Sun
J,
Dean
B and
Scarr
E
(2016). Gene expression changes in the rat frontal cortex caused by sex steroid hormones.
Conference Abstract:
14th Meeting of the Asian-Pacific Society for Neurochemistry.
doi: 10.3389/conf.fncel.2016.36.00057
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Received:
04 Aug 2016;
Published Online:
11 Aug 2016.
*
Correspondence:
Dr. Andrea Gogos, Florey Institute of Neuroscience and Mental Health, Biological Psychiatry and Mental Health Division, Parkville, VIC, Australia, andrea.gogos@florey.edu.au