Innate and acquired immunity and apoptosis of lymphocytes and granulocytes in rabbits experimentally infected with RHDV
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1
University of Szczecin, Faculty of Biology, Department of Immunology, Poland
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2
University of Szczecin, Faculty of Biology, Department of Microbiology, Poland
RHDV is a factor causing viral haemorrhagic disease, being a appropriate model for hemorrhagic fevers and hepatitis in humans. This paper presents an immunological picture in 30rabbits (10infected with RHDV strain-237/04, 10infected with an antigenic variant RHDVa-72-V/2003 and 10control) infected with RHDV. The blood was sampled from the marginal ear vein at 0,8,12,24h p.i.
The innate immunological picture was determined by the ability of adherence, absorption (absorption index, %absorbing cells), NBT test (spontaneous, stimulated, spectrophotometric, stimulation index), myeloperoxidase activity in PMN cells, the concentration and activity of lysozyme in the serum and the percentage of T-cells with receptor CD8+, while acquired immunity was determined by percentage of monostained T-cells with receptor CD5+, CD4+ and CD25+, and of B-cells with receptor CD19+. The apoptosis of lymphocytes and granulocytes was determined by the total caspase1-9 activity.
Studies have shown that the strain 237/04 of RHDV in comparison to strain 72-V/2003-an antigenic variant (RHDVa), provides more than 40%changes in studied parameters. Those changes with the RHDV strain were recorded as increases and decreases in the 8,12,24h of study, and changes with RHDVa, were registered mostly as decreases in 12,24h and rarely in 8h (CD8+). The apoptosis of lymphocytes and granulocytes in case of RHDV incorporated at 8,12,24h, and in case of RHDVa at 12,24h.
Acknowledgements
The paper was financed from scientific grant from Ministry of Science and Higher Education in Poland no. N308289937
Keywords:
innate immunity,
Acquired Immunity,
Apoptosis,
rabbit,
leukocyte
Conference:
15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.
Presentation Type:
Abstract
Topic:
Host-pathogen interactions
Citation:
Adamiak
M,
Tokarz-Deptuła
B,
Trzeciak-Ryczek
A,
Działo
J,
Hukowska-Szematowicz
B,
Niedźwiedzka-Rystwej
P and
Deptuła
W
(2013). Innate and acquired immunity and apoptosis of lymphocytes and granulocytes in rabbits experimentally infected with RHDV.
Front. Immunol.
Conference Abstract:
15th International Congress of Immunology (ICI).
doi: 10.3389/conf.fimmu.2013.02.01102
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Received:
11 Jul 2013;
Published Online:
22 Aug 2013.
*
Correspondence:
Prof. Wiesław Deptuła, University of Szczecin, Faculty of Biology, Department of Microbiology, Szczecin, Poland, kurp13@univ.szczecin.pl