The vitamin D and expression of IL-10 and NF-KB genes in Iranian multiple sclerosis patients
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1
tarbiat modares university, Iran
Background: Multiple sclerosis (MS) is a complex autoimmune disease of the central nervous system characterized by chronic inflammation, demyelination, and axonal damage. Despite decades of research, the etiology and pathogenesis of MS is still unknown, though both genetic and environmental components of the disease seem evident. One potential environmental factor affecting the development of MS may be vitamin D [1,25-(OH)2D3]. A protective effect of Vitamin D on multiple sclerosis is supported by the reduced risk associated with sun exposure and use of Vitamin D supplements. Moreover, high circulating levels of Vitamin D have been associated with lower risk of multiple sclerosis. We hypothesize that 1,25 dihydroxyvitamin D3 may regulate gene expression patterns in a manner that would resolve inflammation.
Methods: To test this hypothesis, Multiple sclerosis patients placed into a vitamin D supplemented group. Blood samples from 10 patients with MS before and 2 months after vitamin D therapy and 10 age and sex-matched controls obtained. Level of the IL-10 & NF-KB mRNA measured using Real Time PCR and the results compared.
Results: we observed that the expression of the IL-10 and NF-KB genes in MS patients after vitamin D therapy increase 3.32 times and 1.6 times respectively.
Conclusion: In summary, this is the first study to report the expression of the IL-10 and NF-KB in MS patients supplemented vitamin D. This study further implicates vitamin D as a strong environmental candidate in MS by demonstrating functional interaction with the effecting genes in MS.
References
1- Gelati M. , Lamperti E. , Dufour A. , Corsini E., Venegoni E. , Milanese C., Nespolo A. , Salmaggi A. 1IL-10 production in multiple sclerosis patients, SLE patients and healthy controls: preliminary findings, ltat, J. Neurol. Sci. 18:191-194, 1997
2- Irfan Rafique and Jette Lautrup Frederiksen, A Complete Literature Survey: Vitamin D and Multiple Sclerosis – What is Clearly Evident? ENJ 2010; 2(2): 113-120.
3-Ascherio A, Munger KL, Simon KC: Vitamin D and multiple sclerosis. Lancet Neurol 2010, 9:599-612.
4-Simpson S Jr, Taylor B, Blizzard L, Ponsonby AL, Pittas F, Tremlett H, Dwyer T, Gies P, van der Mei I: Higher 25-hydroxyvitamin D is associated with lower relapse risk in multiple sclerosis. Ann Neurol 2010, 68:193-203.
Keywords:
Multiple Sclerosis,
Vitamin D,
IL-10,
NF-kB,
autoimmune disease
Conference:
15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.
Presentation Type:
Abstract
Topic:
Immune-mediated disease pathogenesis
Citation:
Shirvani Farsani
Z and
Behmanesh
M
(2013). The vitamin D and expression of IL-10 and NF-KB genes in Iranian multiple sclerosis patients.
Front. Immunol.
Conference Abstract:
15th International Congress of Immunology (ICI).
doi: 10.3389/conf.fimmu.2013.02.01081
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Received:
30 Jun 2013;
Published Online:
22 Aug 2013.
*
Correspondence:
Mrs. Zeinab Shirvani Farsani, tarbiat modares university, Tehran, Iran, zbshirvani@gmail.com