Role of cutatneous regulatory T cells in regulation of immune homeostasis and contact hypersensitivity reaction in the skin
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1
Kyoto university Graduate School of Medicine, Department of Dermatology, Japan
There is accumulating evidence that CD4+Foxp3+ regulatory T (Treg) cells actively suppress pathological and physiological immune responses, thereby contributing to the maintenance of immunological self-tolerance and immune homeostasis and to suppressive control of a broad spectrum of immune responses. We and others have previously shown that there are a substantial number of Treg cells in the skin even in a steady state. However, it remains unclear whether Treg cells in non-immunized skin contribute to the maintenance of immune homeostasis and the suppression of antigen-specific immune responses, e.g., contact hypersensitivity (CHS). To address this issue, we investigated whether the selective elimination of cutaneous Treg cells would lead to the dysregulation of immune homeostasis in the skin and exacerbate CHS reaction. Intradermal injection of an immunotoxin targeting human CD2 (hCD2) into ear skin of Foxp3hCD2 mice, in which Treg cells specifically express hCD2 on their cell surface, markedly reduced Treg cells only in the treated skin, but neither in other organs nor in the blood. Selective depletion of Treg cells in the skin led to the spontaneous development of inflammation in the treated skin. In addition, CHS reaction elicited at the Treg cell-depleted ear was exacerbated and prolonged, accompanied by increase of immune cell invasion and overproduction of Th1- and Th2-related cytokines. These results indicate that Treg cells existing in the skin are essential for the proper regulation of immune homeostasis and antigen-specific immune response in the skin.
Keywords:
Regulatory T Cell,
Skin Diseases,
Homeostasis,
Contact hypersensitivity,
mouse models
Conference:
15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.
Presentation Type:
Abstract
Topic:
Innate immunity
Citation:
Hanakawa
S,
Kitoh
A,
Miyachi
Y and
Kabashima
K
(2013). Role of cutatneous regulatory T cells in regulation of immune homeostasis and contact hypersensitivity reaction in the skin.
Front. Immunol.
Conference Abstract:
15th International Congress of Immunology (ICI).
doi: 10.3389/conf.fimmu.2013.02.01038
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Received:
11 Jul 2013;
Published Online:
22 Aug 2013.
*
Correspondence:
Dr. Kenji Kabashima, Kyoto university Graduate School of Medicine, Department of Dermatology, Kyoto, Kyoto, 606-8507, Japan, kaba@kuhp.kyoto-u.ac.jp