Implication of gamma delta T cells in the immune response against murine CMV
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1
CNRS, Immunology, France
We previously showed that gamma delta T cells are key contributors to the immune response against CMV in humans, and participate to CMV riddance partially through interferon gamma (IFNg) release. The aim of the present study was to use the mouse model of CMV infection in order to dissect in vivo the implication of murine gamma delta T cells in the control of mouse CMV (MCMV). Using C57BL/6 mice deficient for alpha beta and/or gamma delta T cells, we showed that whichever subpopulation is sufficient to confer protection against MCMV. Viral loads increased with time in all organs tested from CD3epsilon-/- (alpha beta- gamma delta-) infected mice, and the presence of high levels of transaminases in blood was suggestive of liver damage. CD3epsilon-/- ultimately died unlike TCRalpha-/- (alpha beta- gamma delta+) mice that survived with much lower viral loads. In TCRalpha-/- mice, CD27+ gamma delta T cells were increased in the liver 7 days post MCMV-infection suggesting an implication of IFNg-secreting gamma delta T cells in the clearance of MCMV in this organ. Finally, bone marrow transfer experiments using TCRalpha-/- donors rendered CD3epsilon-/- mice resistant to MCMV in accordance with a protective anti-viral role for gamma delta T cells. These results which suggest that gamma delta T cells could compensate for the absence of alpha beta T cells during MCMV infection could be of particular relevance in immune-suppressive contexts where alpha beta T cells are more specifically compromised.
Keywords:
Gamma Delta T cells,
CMV infection,
CD27,
IFN- γ,
mouse model
Conference:
15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.
Presentation Type:
Abstract
Topic:
Innate immunity
Citation:
Khairallah
C,
Netzer
S,
Villacreces
A,
Juzan
M,
Praloran
V,
Moreau
J,
Déchanet-Merville
J and
Capone
M
(2013). Implication of gamma delta T cells in the immune response against murine CMV.
Front. Immunol.
Conference Abstract:
15th International Congress of Immunology (ICI).
doi: 10.3389/conf.fimmu.2013.02.00948
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Received:
28 Jun 2013;
Published Online:
22 Aug 2013.
*
Correspondence:
Dr. Myriam Capone, CNRS, Immunology, Bordeaux, 33076, France, mcapone@cirid.org