MASP2 and MBL: Dynamics and Intrathecal synthesis
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1
Laboratorio Central de Líquido Cefalorraquídeo (LABCEL)Universidad de Ciencias Médicas de La Habana, Cuba
Alberto Juan Dorta Contreras1, Barbara Padilla Docal1, Oliver Pérez Martín2, Asdrubal Arias Morales1, Hansotto Reiber3, Jens Christian Jensenius4
1Labcel, Medical Sciences University Havana, Havana, Cuba 2 Finlay Institute Havana, Cuba 3Neurochemistry Laboratory, University Göttingen, Göttingen, Germany 4Department of Biomedicine, University of Aarhus, Aarhus, Denmark
Background. There is an increasing interest to look for the possible participation in lectin pathway components in the immune response in CNS.
Methods
MASP2 and MBL was assayed in 20 control samples of CSF and serum with an ELISA, coated with anti MASP2 and MBL antibodies. Routine parameters such as albumin-, immunoglobulin- CSF/serum quotients, oligoclonal IgG and cell count were used to characterize the patient groups. Groups comprised firstly, control patients without organic brain disease with normal CSF and normal barrier function and secondly, patients without inflammatory diseases but with increased QAlb, i.e. with a blood CSF barrier dysfunction.
Results
MASP2 concentration in CSF was at least two-fold higher than expected for a molecular-size-dependent passage from blood and CSF MBL was at least five-fold 2) in a QMASP2 vs QAlb regression line 9/20 cases showed an intrathecal fraction and QIgM/QAlb quotient diagram (Reibergram) 9/13 cases showed an intrathecal fraction in some cases over 80% of total CSF MBL concentration 3) The higher inter-individual variation of MASP2 concentrations in CSF of the control group (CV = 55%) compared to the MASP2 concentrations in serum (CV = 40%) indicate that MASP2 is mostly a blood protein and the smaller inter-individual variation of CSF MBL of the control group compared to serum MBL concentration indicate an independent source of CSF MBL 4) The absolute MASP2 and MBL concentration in CSF increases with increasing QAlb.
Conclusions
MASP2 in CSF is predominantly blood-derived. MBL dynamics indicate that only the leptomeningeal proteins shower a linear increase with decreasing CSF flow rate.
Keywords:
MASP2,
Innate immunology,
Lectin pathway,
Cerebrospinal Fluid,
blood brain barrier,
protein diffusion
Conference:
15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.
Presentation Type:
Abstract
Topic:
Innate immunity
Citation:
Dorta Contreras
A
(2013). MASP2 and MBL: Dynamics and Intrathecal synthesis.
Front. Immunol.
Conference Abstract:
15th International Congress of Immunology (ICI).
doi: 10.3389/conf.fimmu.2013.02.00929
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Received:
26 Jun 2013;
Published Online:
22 Aug 2013.
*
Correspondence:
Prof. Alberto Juan Dorta Contreras, Laboratorio Central de Líquido Cefalorraquídeo (LABCEL)Universidad de Ciencias Médicas de La Habana, Havana, Cuba, adorta@infomed.sld.cu