Impact of vaccination route and chosen adjuvant on immune responses and host-pathogen interplay during influenza infection.
Vanessa
Zurli1, 2,
Marianna
Taccone1,
Michela
Brazzoli1,
Simona
Gallorini3,
Caterina
Galeone4,
Alessandra
Bonci4,
Daniele
Casini4,
Barbara
Baudner3,
Ennio
De Gregorio1,
Sylvie
Bertholet1 and
Anja
Seubert1*
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1
Novartis Vaccines & Diagnostics, Immunology, Italy
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2
Università degli Studi di Padova, Dipartimento di Biologia, Italy
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3
Novartis Vaccines & Diagnostics, Formulation, Italy
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4
Novartis Vaccines & Diagnostics, Serology, Italy
Influenza causes an acute infection in the host and initiates a cascade of immune reactions activating almost all components of the immune system. Most of the initial innate response, including cytokine release, influx of granulocytes or natural killer cells and cell activation, is responsible for the acute onset of the clinical symptoms. On the other hand, innate immunity is an essential prerequisite for the development of adaptive immune responses leading to disease resolution.
In this study, we evaluated the immunological response to influenza virus A/PR/8/34 (H1N1) infection of naïve Balb/c mice in comparison to virus pre-exposed or differently immunized mice. Using a panel of different vaccination routes and adjuvants, we characterized the impact of different vaccination-induced immune responses during actual pathogen encounter, and how it contributes to disease resolution or pathology.
We immunized Balb/c mice systemically or mucosally with an influenza subunit vaccine (HA/NA proteins from A/California/7/2009/H1N1) formulated with i) MF59 to induce a mixed Th1/Th2 response, ii) a combination of MF59 and CpG to get a more Th1-prone response and iii) LTK63 to obtain a Th1/Th17 polarized response. We assessed antibody titers and individual T helper profiles after vaccination and dissected the complex host-pathogen interplay at different timepoints after infection. To that end, we determined ex-vivo CD4 T helper responses in the lung, characterized differential lung cell composition and cytokine environments.
Our studies should help to better understand how immune responses and ultimately vaccination success can be steered via different administration routes and adjuvant formulations.
Keywords:
influenza,
T helper cells,
adjuvant,
lung recruitment,
Cytokines
Conference:
15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.
Presentation Type:
Abstract
Topic:
Host-pathogen interactions
Citation:
Zurli
V,
Taccone
M,
Brazzoli
M,
Gallorini
S,
Galeone
C,
Bonci
A,
Casini
D,
Baudner
B,
De Gregorio
E,
Bertholet
S and
Seubert
A
(2013). Impact of vaccination route and chosen adjuvant on immune responses and host-pathogen interplay during influenza infection..
Front. Immunol.
Conference Abstract:
15th International Congress of Immunology (ICI).
doi: 10.3389/conf.fimmu.2013.02.00900
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Received:
19 Jun 2013;
Published Online:
22 Aug 2013.
*
Correspondence:
Mrs. Anja Seubert, Novartis Vaccines & Diagnostics, Immunology, Siena, 53100, Italy, anja.seubert@novartis.com