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Mannan-binding lectin inhibits Candida albicans-induced cellular responses of human THP-1 cells through toll-like receptor 2 and toll-like receptor 4

Mingyong Wang1*, Fanping Wang1, Wen-jun Wang2, Jian-bin Yang1, Dong-fang Zhao1, Hong-po Wang1, Feng Shao1, Rui-li Sun1, Zhai Jing-jing1 and Shi-jun Song1
  • 1 Xinxiang Medical University, China
  • 2 Southern Medical University, China

Background
Candida albicans (C. albicans), the most common human fungal pathogen, can cause fatal systemic infections under certain circumstances. Mannan-binding lectin (MBL) ，a member of the collectin family in the C-type lectin superfamily, is an important serum component associated with innate immunity. Toll-like receptors (TLRs) are expressed extensively, and have been shown to be involved in C. albicans-induced cellular responses. We first examined whether MBL modulated heat-killed (HK) C. albicans-induced cellular responses in the monocytoid cell line, THP-1 cells. We then investigated the possible mechanisms of its inhibitory effect.
Results
Enzyme-linked immunosorbent assay (ELISA) and reverse transcriptasepolymerase chain reaction (RT-PCR) analysis showed that MBL at higher concentrations (10-20 µg/ml) significantly attenuated C. albicans-induced proinflammatory cytokine (e.g., TNF-α) and chemokine (e.g., IL-8) production from THP-1 cells at both protein and mRNA levels. Electrophoretic mobility shift assay (EMSA) and Western blot (WB) analysis showed that MBL could inhibit C. albicans-induced NF-κB DNA binding and its translocation in THP-1 cells. Furthermore, MBL could directly bind to C. albicans in a Ca2+-dependent manner and decrease TLR2 and TLR4 expressions in C. albicans-induced THP-1 cells.
Conclusion
All these data suggest that MBL could affect cytokine expression by modifying C. albicans-/TLR-signaling pathways. This study supports an important role for MBL on the regulation of C. albicans-induced cellular responses.

Acknowledgements

Very special thanks should be given to professors Tian-Yun Wang, Wen-Ming Yong for their dedicated revision of the paper.

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Keywords: Mannan-binding lectin, Candida albicans, Monocytes, Cytokines, Toll-Like Receptors, Nuclear factor-κB

Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.

Presentation Type: Abstract

Topic: Innate immunity

Citation: Wang M, Wang F, Wang W, Yang J, Zhao D, Wang H, Shao F, Sun R, Jing-jing Z and Song S (2013). Mannan-binding lectin inhibits Candida albicans-induced cellular responses of human THP-1 cells through toll-like receptor 2 and toll-like receptor 4. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00893

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Received: 23 Jun 2013; Published Online: 22 Aug 2013.

* Correspondence: Dr. Mingyong Wang, Xinxiang Medical University, Xinxiang, China, wmy118@126.com