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Effect of Entamoeba histolytica calreticulin in the immune response of patients with acute amoebic liver abscess

Miriam Nieves1, Rivas Enrique1, Moran Patricia1, Valadez Alicia1, Garcia Maria Del Carmen1, Rojas Liliana1, Oswaldo Partida-Rodriguez1, Hernandez Eric1, Cerritos Rene1 and Ximenez Cecilia1*
  • 1 NATIONAL AUTONOMOUS UNIVERSITY OF MEXICO, DEPARMENT OF EXPERIMENTAL, FACULTY OF MEDICINE, Mexico

Entamoeba histolytica is a human intestinal parasite that causes amoebiasis, which in Mexico is considerate as an endemic disease. Some molecules are involved in pathogenicity and virulence of E. histolytica. It was previously reported that E. histolytica calreticulin (EhCRT) is a highly immunogenic protein in patients with amoebiasis. The aims of this work were to characterize the isotype of antibody response in serum samples of patients during the acute phase of amoebic liver abscess (ALA), to measure the ability of EhCRT to bind human C1q protein and to define the stimulation properties of EhCRT upon peripheral mononuclear blood cells (PMBC). We compared individuals with ALA and healthy subjects, which were characterized by coproparasitoscopic and ELISA assays and took a peripheral blood sample to obtain serum and mononuclear cells. PMBC were isolated and cultured in an in vitro system and stimulated with recombinant rEhCRT. The stimulation index was calculated. Results show that the major isotypes of serum antibodies against EhCRT were IgM and IgG class. Levels of IgA in serum were low and levels of IgE isotype of antibodies were extremely low. Besides, rEhCRT-C1q caused the inhibition of classical complement pathway in vitro. PMBC of patients with ALA display a comparatively higher stimulation index to control PMBC. These preliminary results suggest that EhCRT can be a useful antigen and have a modulating role to the adaptive immune response in invasive amoebiasis.

Acknowledgements

Work performed with support PAPIIT/UNAM: IN226511 and CONACYT 210CO1-140990.

Keywords: Entamoeba histolytica, Carleticulin, Amoebic liver abscess, peripheral mononuclear blood cells, human C1q protein

Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.

Presentation Type: Abstract

Topic: Host-pathogen interactions

Citation: Nieves M, Enrique R, Patricia M, Alicia V, Maria Del Carmen G, Liliana R, Partida-Rodriguez O, Eric H, Rene C and Cecilia X (2013). Effect of Entamoeba histolytica calreticulin in the immune response of patients with acute amoebic liver abscess. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00763

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Received: 17 Jun 2013; Published Online: 22 Aug 2013.

* Correspondence: Prof. Ximenez Cecilia, NATIONAL AUTONOMOUS UNIVERSITY OF MEXICO, DEPARMENT OF EXPERIMENTAL, FACULTY OF MEDICINE, MEXICO CITY, Mexico, cximenez@servidor.unam.mx