Roles of basophils and mast cells infiltrating the lung in murine asthmatic responses induced by multiple antigen challenges
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1
Kyoto Pharmaceutical University, Pharmacology, Japan
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2
Kyoto Pharmaceutical University, Pathological Biochemistry, Japan
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3
Kobe Pharmaceutical University, Pharmacology, Japan
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4
University of Alabama at Birmingham, Microbiology, United States
Mast cell hyperplasia has been observed in the lungs of mice with experimental asthma, but few reports have studied basophils. Here, we discriminated and quantified mast cells and basophils in the lungs in a murine asthma model, determining if both cells were increased by multiple antigen challenges, and assessing the roles of these cells in asthmatic responses. Sensitized Balb/c mice were challenged intratracheally with ovalbumin 4 times. Mast cells and basophils in enzymatically digested lung tissue were detected by flow cytometry. An anti-FcεRI monoclonal antibody, MAR-1, was administered i.p. during the multiple challenges. The numbers of both mast cells (IgE+ C-kit+) and basophils (IgE+ C-kit− CD49b+) increased in the lungs after three challenges. Treatment with MAR-1 completely abolished the increases; however, a late-phase increase in specific airway resistance (sRaw), and airway eosinophilia and neutrophilia were not affected by the treatment, although the early-phase increase in sRaw was suppressed. MAR-1 reduced antigen-induced airway interleukin (IL)-4 production. Basophils infiltrating the lung clearly produced IL-4 after antigen stimulation in vitro; however, histamine and murine mast cell protease 1 were not increased in the serum after the challenge, indicating that mast cell activation was not evoked. In conclusion, both mast cells and basophils infiltrated the lungs by multiple intratracheal antigen challenges in sensitized mice. Neither mast cells nor basophils were involved in late-phase airway obstruction in this model, although early-phase airway obstruction was mediated by basophils. Targeting basophils in asthma therapy may be useful for an early asthmatic response.
Keywords:
allergy,
Asthma,
Basophils,
Mast Cells,
IL-4
Conference:
15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.
Presentation Type:
Abstract
Topic:
Innate immunity
Citation:
Kida
H,
Takiguchi
A,
Wakamori
H,
Ishihara
K,
Akiba
S,
Mizutani
N,
Yoshino
S,
Chaplin
DD,
Ohya
S and
Nabe
T
(2013). Roles of basophils and mast cells infiltrating the lung in murine asthmatic responses induced by multiple antigen challenges.
Front. Immunol.
Conference Abstract:
15th International Congress of Immunology (ICI).
doi: 10.3389/conf.fimmu.2013.02.00745
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Received:
15 Jun 2013;
Published Online:
22 Aug 2013.
*
Correspondence:
Dr. Takeshi Nabe, Kyoto Pharmaceutical University, Pharmacology, Kyoto, 607-8414, Japan, nabe@mb.kyoto-phu.ac.jp