INFLUENCE OF TIM-1 HAPLOTYPES ON CD4 COUNTS IN HIV SEROPREVALENT NORTH INDIANS
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1
All India Institute of Medical Sciences, Department of Transplant Immunology and Immunogenetics, India
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2
Tokyo Medical and Dental University, Department of Molecular Pathogenesis, Medical Research Institute, Japan
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3
All India Institute of Medical Sciences, Department of Medicine, India
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4
All India Institute of Medical Sciences, Department of Microbiology, India
Introduction: T cell immunoglobulin and mucin domain family member-1 (TIM-1) molecules are expressed on Th2 CD4+ cells and regulate Th1/Th2 immune responses. The mucin domain (exon 4) of this molecule is characterized by high degree of variability. However, the distribution of TIM-1 haplotypes and their population specific influence on HIV/AIDS is largely unknown.
Material & Methods: Sequencing analysis of TIM-1 exon 4 was performed in 227 HIV +ve and 288 healthy individuals (North Indians). Haplotypes were compared among the groups by the chi square test. Continuous variables with the haplotypic background were compared by Wilcoxon rank-sum test.
Results: Seven TIM-1 haplotypes W-A, W-C, D1, D1*, D3-A, D3-C and D4 were observed. D3-A was observed predominantly (>0.465) followed by D3-C (0.219), D4 (0.214), W-A (0.057), D1 (0.021) and W-C (0.007) haplotypes. Differences among the healthy vs HIV +ve individuals were statistically insignificant, suggesting lack of influence on HIV susceptibility/resistance. Additionally, a novel D1 related haplotype D1* was observed (0.007). Further, significantly higher CD4 counts were observed in D3-A carrying HIV patients, however in subgroups of ART naïve and on ART patients, moderate influence was observed. These findings suggest a possible link of D3A with low levels of TIM-1 expression and hence lower Th2 promotion and enhanced Th1 responses. Incidentally, Th1 to Th2 shift is well associated with poor HIV prognosis.
Conclusion: We represent the first report on distribution of TIM-1 haplotypes in the North Indian population in context of HIV susceptibility/resistance and disease status.
Supported By: Indo-Japan (DST-JSPS) project (DST/INT/JAP/P-74/2009)
Acknowledgements
We acknowledge Dr. Toshiaki Nakajima for his support in the study. We are thankful to the India-Japan Cooperative Science Program from Japan Society for the Promotion of Science (JSPS), Japan and Department of Science and Technology (DST), India and research grants from the Ministry of Health, Labor and Welfare, Japan, and a support for women researchers from the Tokyo Medical and Dental University.
Keywords:
TIM-1,
Haplotypes,
HIV,
aids,
Art
Conference:
15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.
Presentation Type:
Abstract
Topic:
Host-pathogen interactions
Citation:
Sharma
G,
Kimura
A,
Kaur
G,
Ohtani
H,
Mehra
N,
Sharma
S and
Vajpayee
M
(2013). INFLUENCE OF TIM-1 HAPLOTYPES ON CD4 COUNTS IN HIV SEROPREVALENT NORTH INDIANS.
Front. Immunol.
Conference Abstract:
15th International Congress of Immunology (ICI).
doi: 10.3389/conf.fimmu.2013.02.00682
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Received:
12 Jun 2013;
Published Online:
22 Aug 2013.
*
Correspondence:
Dr. Gaurav Sharma, All India Institute of Medical Sciences, Department of Transplant Immunology and Immunogenetics, New Delhi, India, gaurav_ash19@yahoo.com