Event Abstract

Back to Event

Effects of Vit D on Th17 cells and related cytokines in lupus erythematosus patients

Hadi Reihani1*, Maryam Rastin1, Maryam Sahebari2, Mahmoud Mahmoudi1, Mohsen Ghoryani1, Nafiseh Abdollahi2, Nafiseh Sadat Tabasi1, Shahrzad Zamani Taghizadeh Rabe1, Fahimeh Lavi Arab1 and Fatemeh Faraji1
  • 1 Immunology Research Center, BuAli Research Institute, Faculty of Medicine, Mashhad University of Medical Sciencses, Mashhad, Iran
  • 2 Rheumatology Research Center, Ghaem Hospital,Mashhad University of Medical Sciencses, Mashhad, Iran

Introduction: Systemic lupus erythematosus is a multisystem inflammatory autoimmune disease characterized by autoreactive T cell activation against selt-antigens and subsequent production of autoantibodies causing inflammation and tissue damage.Emerging data suggests that Th17 cells are the main pathogenic cells involed in SLE that secret proinflamatory cytokines and increased frequency of these cells in peripheral blood is correlated with disease activity. Environmental and genetic factors are involved in the pathogenesis of SLE disease. Vitamin D deficiency has been proposed as a potential environmental factor triggering SLE. In present study we investigated the effects of vitamin D on modolution of Th17 cells and related cytokines.
Material and methods: Blood samples were obtained from 30 patients with SLE. Lymphocytes isolated using Ficoll-Hypaque. Isolated lymphocytes were cultured in the presence and in the absence of vitamin D. After incubation the IL-17 secreting cells were analyzed by flowcytometry, and also RNA extracted using Trizol, cDNA synthesized, and the expression levels of IL-17, IL-6, IL-23 genes was analyzed by Real-time PCR.
Results: 1,25 dihydroxyvitamin D decreased IL-17 secreting cells ,and the expression levels of IL-17,IL-6, IL-23 genes in SLE patients.

References

1- Vieira VM, Hart JE, Webster TF,Weinberg J, Puett R,Laden F, et al. Association between residences in U.S. northern latitudes and rheumatoid arthritis: a spatial analysis of the Nurses’ Health Study. Environmental Health Perspectives. 2010;118(7):957-61.
2- Holick MF . Vitamin D: a millennium perspective. Journal of Cellular Biochemistry. 2003;88:296-307.
3- Pelajo CF , Lopez-Benitez JM, Miller LC. Vitamin D and autoimmune rheumatologic disorders. Autoimmunity Reviews. 2010;9(7):507-10.
4- Shoenfeld N , Amital H,S hoenfeld Y. The effect of melanism and vitaminD synthesis on the incidence of autoimmune disease. Nat Clin Pract Rheumatol. 2009;5:99-105.
5- Adorini L. Intervention in autoimmunity: the potential of vitamin D receptor agonists. Cellular Immunology. 2005;233:115-24.
6- Orbach H, Zandman-Goddard G, Amital H, Barak V, Szekanecz Z, Szucs G, et al Novel biomarkers in autoimmune diseases: prolactin, ferritin, vitaminD, and TPA levels in autoimmune diseases. Ann NY Acad Sci. 2007;1109:385-400.
7- Veldman CM , Cantorna MT, DeLuca HF. Expression of 1,25- Dihydroxy vitamin D(3) receptor in the immune system. Arch Biochem Biophys. 2000;374(2):334-8.
8- Takahashi K, Nakayama Y, Horiuchi H, Ohta T, Komoriya K, Ohmori H, Kamimura T. Human neutrophils express messenger RNA of vitamin D receptor and respond to 1 alpha, 25-Dihydroxy vitamin D3. Immunopharmacol Immunotoxicol. 2002;24(3):335-47.
9- Gauzzi MC , Purificato C, Donato K, Jin Y, Wang L, et al. Suppressive effect of 1 alpha, 25-dihydroxy vitamin D3 on type I IFN-mediated monocyte differentiation into dendritic cells: impairment of functional activities and chemotaxis. The Journal of Immunology. 2005;174:270-6.
10- Helming L, Bose J, Ehrchen J, Schiebe S, Frahm T, Geffers R, et al. 1 alpha, 25-Dihydroxy vitamin D3 is a potent suppressor of interferon gamma- mediated macrophage activation. Blood. 2005;106(13):4351-8.
11- Linker-Israeli M, Elstner E, Klinenberg JR, Wallace DJ, Koeffler HP. Vitamin D(3) and its synthetic analogs inhibits the spontaneous in vitro immunoglobulin production by SLE-derived PBMC. Clin Immunol. 2001;99(1):82-93.
12- Mahon B.D,Wittke A,Weaver.V, et al. The targets of vitamin D depend on the differentiation and activation status of CD4 positive T cells. Cell Biochem. 2003;89:922-32.
13- Steinman L. A brief history of T(H)17,the first major revision in the T(H)1/T(H)2 hypothesis of T cell-mediated tissue damage. Nat Med. 2007;13(2):139-45.
14- Dardalhon V, Korn T, Kuchroo VK , Anderson AC. Role of Th1 and Th17 cells in organ-specific autoimmunity. J Autoimmun. 2008;31:252-6.
15- Yang J, Chu Y, Yang X,Gao D,et al. Th17 and natural Treg cell population dynamics in systemic lupus erythematosus. Arthritis and Rheumatism. 2009;60(5):1472-83.
16- Kleczynska W, Jakiela B, Plutecka H,Milewski M,Sanak M,Musial J. Imbalance between Th17 and regulatory T-cells in systemic lupus erythematosus. Folia Histochemica Et Cytobiologica. 2011;49(4):646-53.
17- Stockinger B. Th17 cells: an orphan with influence. Immunol Cell Biol. 2007;85(2):83.
18- Arnson Y, Shoenfeld Y. Vitamin D and autoimmunity : new aetiological and therapeutic considerations. Ann Rheum Dis. 2007;66:1137-42.
19- Penna G, Adorini L. 1 Alpha, 25-dihydroxy vitamin D3 inhibits differentiation, maturation, activation, and survival of dendritic cells leading to impaired alloreactive Tcell activation. J Immunol. 2000;164(5):2405-11.
20- Kwan BCH, Tam LS, Lai KB,Lai FMM,et al. The gene expression of type 17 T-helper cell-related cytokines in the urinary sediment of patients with systemic lupus erythematosus. 2009;48:1491-7.
21- Palmer MT, Lee YK,Maynard CL,Oliver JR,Bikle DD,Jetten AM,Weaver CT. Lineage-specific effects of 1,25-dihydroxyvitamin D3 on the development of effector CD4 T cells. The Journal Of Biological Chemistry. 2011;286(2):997-1004.
22- Xing Q, Wang B, Su H, Cui J, Li J. Elevated Th17 cells are accompanied by FoxP3+ Treg cells decrease in patients with lupus nephritis.Rheumatol Int. 2011;1771:296-310|
23- Shah K, Lee WW, Lee SH, Kim SH, Kang SW, Craft J, Kang I. Dysregulated balance of Th17 and Th1 cells in systemic lupus erythematosus. Arthritis Res Ther. 2010;12(2):R53.
24- Hedrich CM , Rauen T, Kis-Toth K, Kyttaris VC, Tsokos GC. cAMP response element modulator (CREM)α suppresses IL-17F expression in T lymphocytes from patients with systemic lupus erythematosus (SLE). J Biol Chem. 2011;287:4715-25
25- Joshi S , Pantalena LC, Liu XK, Gaffen SL, Liu H, Rohowsky-Kochan C, Ichiyama K, Yoshimura A, Steinman L, Christakos S, Youssef S. 1,25-dihydroxyvitamin D(3) ameliorates Th17 autoimmunity via transcriptional modulation of interleukin-17A. Mol Cell Biol. 2011;31(17):3653-69.

Keywords: SLE, Th17, Vitamin D, IL-17, IL-6, IL-23

Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.

Presentation Type: Abstract

Topic: Immune-mediated disease pathogenesis

Citation: Reihani H, Rastin M, Sahebari M, Mahmoudi M, Ghoryani M, Abdollahi N, Tabasi N, Zamani Taghizadeh Rabe S, Lavi Arab F and Faraji F (2013). Effects of Vit D on Th17 cells and related cytokines in lupus erythematosus patients. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00597

Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters.

The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated.

Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed.

For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions.

Received: 22 Apr 2013; Published Online: 22 Aug 2013.

* Correspondence: Mr. Hadi Reihani, Immunology Research Center, BuAli Research Institute, Faculty of Medicine, Mashhad University of Medical Sciencses, Mashhad, Mashhad, Iran, reihanih1@mums.ac.ir