Commensal microbiota monitoring by RLRs regulates contact hypersensitivity response
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1
Centre International de Recherche en Infectiologie (CIRI), INSERM U1111- CNRS UMR5308, France
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2
Molecular Signaling and Cell Death Unit, Department for Molecular Biomedical Research, VIB, Belgium
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3
Laboratory of Experimental Immunology, Center for Cancer Research, NCI/NIH, United States
RIG-I like receptors (RLRs) play a major role in response to cytosolic viral RNAs by initiating an antiviral immune response through the recruitment of the mitochondrial adaptor protein MAVS. A novel regulatory role of the RLRs pathway in sterile inflammation has been recently identified in a murine model of experimental colitis. Using another sterile inflammation model, Contact Hypersensitivity (CHS) to the strong hapten 2,4-dinitrofluorobenzen (DNFB), we showed that MAVS-deficient mice developed an exacerbated response. We characterized this inflammatory response at the challenge site of MAVS-deficient mice and demonstrated a higher oedema due to an increase of immune cell infiltrate with higher proportion of cytotoxic CD8+ T cells and an enhanced production of inflammatory cytokines. As it has been suggested that the monitoring of commensal bacteria by RLRs may be involved in the regulation of intestinal inflammation, we evaluated the role of the microbiota in the enhanced CHS response in MAVS-deficient mice. We performed cohousing experiments and showed that the inflammatory phenotype of MAVS-deficient mice can be transferred to WT mice. Moreover, antibiotic treatment of MAVS-deficient mice strongly inhibited the increased CHS response, confirming the importance of commensal microbiota in the regulation of CHS. Altogether, our results highlight that monitoring of commensal microbiota by RLRs is involved in the regulation sterile inflammatory CHS response.
Keywords:
MAVS,
RIG-I like receptors,
microbiota,
Sterile Inflammation,
Contact hypersensitivity
Conference:
15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.
Presentation Type:
Abstract
Topic:
Innate immunity
Citation:
Plantamura
E,
Moudombi
L,
Djebali
S,
Macari
C,
Lippens
S,
Dzutsev
A,
Trinchieri
G,
Marvel
J and
Michallet
M
(2013). Commensal microbiota monitoring by RLRs regulates contact hypersensitivity response.
Front. Immunol.
Conference Abstract:
15th International Congress of Immunology (ICI).
doi: 10.3389/conf.fimmu.2013.02.00582
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Received:
23 Apr 2013;
Published Online:
22 Aug 2013.
*
Correspondence:
Mrs. Emilie Plantamura, Centre International de Recherche en Infectiologie (CIRI), INSERM U1111- CNRS UMR5308, LYON, 69365 Cedex 07, France, emilie.plantamura@inserm.fr