Potential Roles For Adipose-Derived Mesenchymal Stem Cells In The Regulation Of The Mucosal Immune System In Aging
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1
The University of Alabama at Birmingham, United States
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2
Nihon University School of Dentistry at Matsudo, Japan
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3
Seems Inc, Japan
Various clinical trials have shown the regenerative capacity of adipose-derived stem cells to differentiate into blood cells, epithelial cells, adipocytes, chondrocytes, and osteoblasts. Age-associated dysregulation of the gastrointestinal (GI) immune system has also been well documented. It has been shown that antigen (Ag)-specific secretory IgA (SIgA) antibody (Ab) responses in the GI tract are impaired in aged mice. In this study, we have examined the potential of adipose-derived mesenchymal stem cells (AMSCs) to restore the GI mucosal immune system in aged mice. One year-old mice were adoptively transferred with AMSCs (2 x 106 /mouse). Eight to ten months later, mice were orally immunized with ovalbumin (OVA, 1 mg) plus cholera toxin (10 μg) as mucosal adjuvant three times at weekly intervals. When fecal extracts and plasma were subjected to OVA-specific ELISA, elevated levels of mucosal SIgA and plasma IgG Ab responses were noted in aged recipients. When OVA-stimulated CD4+ T cell cytokine production was examined, Peyer’s patch CD4+ T cells produced increased levels of IL-4. Further, CD4+ T cells from GI tract lamina propria revealed elevated levels of IFN-γ and IL-4 production. In contrast, aged mice without AMSC transfer showed essentially no OVA-specific Ab or CD4+ T cell cytokine responses. Interestingly, total numbers of thymocytes and the frequency of double negative cells were increased in aged mice with AMSC transfer when compared with aged mice without AMSC transfer. These results show major roles for AMSCs in restoring impaired mucosal immunity and the thymus in aged mice.
Keywords:
mucosal immunology,
Mesenchymal Stem Cells,
Aging,
oral immunization,
antigen-specific immune responses
Conference:
15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.
Presentation Type:
Abstract
Topic:
Translational immunology and immune intervention
Citation:
Aso
K,
Kimura
K,
Takagaki
K,
Kobayashi
T,
Oki
K,
Sugimoto
S,
Tanemura
H,
Urushihata
N,
McGhee
JR and
Fujihashi
K
(2013). Potential Roles For Adipose-Derived Mesenchymal Stem Cells In The Regulation Of The Mucosal Immune System In Aging.
Front. Immunol.
Conference Abstract:
15th International Congress of Immunology (ICI).
doi: 10.3389/conf.fimmu.2013.02.00510
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Received:
11 Jun 2013;
Published Online:
22 Aug 2013.
*
Correspondence:
Dr. Kohtaro Fujihashi, The University of Alabama at Birmingham, Birmingham, United States, kohtarof@uab.edu