Identification of NETs (neutrophil extracellular traps) in tegumentary lesions of patients with paracoccidioidomycosis
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1
Universidade Estadual Paulista "Júlio de Mesquita Filho" - UNESP, Depto. Patologia, Brazil
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2
Universidade Estadual Paulista "Júlio de Mesquita Filho" - UNESP, Depto. Microbiologia e Imunologia, Brazil
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3
Universidade Estadual Paulista "Júlio de Mesquita Filho" - UNESP, Depto. Dermatologia e Radioterapia, Brazil
Paracoccidiodomycosis is a systemic mycosis caused by Paracoccidioides brasiliensis (Pb), which is endemic in Latin America. In recent years, works in paracoccidioidomycosis have focused the role of neutrophils (PMNs), since the literature has demonstrated the dynamic involvement of these cells in host defense against various microorganisms. Recent studies have shown that neutrophils may use a third strategy to destroy microorganisms called NETosis, a type of neutrophil death, reported to be distinct from apoptosis, phagocytosis-induced cell death, and necrosis. This cell death pathway involves the release of extracellular traps by activated neutrophils, known as neutrophil extracellular traps (NETs). The NETs are composed by fibers containing decondensed chromatin, histones and several other granular proteins such as elastase, which in addition to ensnare and/or kill microorganisms, increase the inflammatory response. It has been proposed that NETs destroy microorganisms that were not phagocytosed by neutrophils, and this could be proposed for the Paracoccidioides brasiliensis, since the fungus yeast can present various sizes and morphologies. In this context, the objective of this study was identify the presence of NETs in vivo, analyzing tegumentary lesions of patients with paracoccidioidomycosis. Tissue section from formalin-fixed biopsies were stained with DAPI, anti-elastase (FITC) and anti-histone (Texas-Red); and analyzed by confocal imunofluorescence microscopy. The images revealed the presence of NETs constituents, nuclear and extracellular localization of DNA stained with DAPI, co-localization of elastase and histone, which was confirmed by the overlay of these 3 stains. Thus, we identified the mechanism of NETosis in paracoccidioidomycosis patient lesions. Financial Support: FAPESP/CNPq/Fundunesp
Acknowledgements
Financial Support: FAPESP 2011/18855-7; CNPq 480486/2011-5; Fundunesp 00637/11-DFP
Keywords:
NETs,
Paracoccidioidomycosis,
tegumentary lesions,
Neutrophils,
Paracoccidioides brasiliensis
Conference:
15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.
Presentation Type:
Abstract
Topic:
Host-pathogen interactions
Citation:
Della Coletta
AM,
Bachiega
TF,
De Quaglia E Silva
JC,
Marques
SA,
Marques
ME,
Defaveri
J,
Soares
AM and
Dias-Melicio
LA
(2013). Identification of NETs (neutrophil extracellular traps) in tegumentary lesions of patients with paracoccidioidomycosis.
Front. Immunol.
Conference Abstract:
15th International Congress of Immunology (ICI).
doi: 10.3389/conf.fimmu.2013.02.00508
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Received:
25 Apr 2013;
Published Online:
22 Aug 2013.
*
Correspondence:
Miss. Amanda M Della Coletta, Universidade Estadual Paulista "Júlio de Mesquita Filho" - UNESP, Depto. Patologia, Botucatu, São Paulo, 18618-970, Brazil, amdcoletta@gmail.com
Prof. Luciane A Dias-Melicio, Universidade Estadual Paulista "Júlio de Mesquita Filho" - UNESP, Depto. Patologia, Botucatu, São Paulo, 18618-970, Brazil, ladiasmelicio@fmb.unesp.br