Tumor associated fibroblasts are endowed with increased ability to support tumor development
-
1
University of Medicine and Pharmacy Victor Babes Timisoara, Functional Sciences, Romania
Tumor associated fibroblasts (TAFs) from solid human tumors provide structural and functional support to tumor development and progression. The main purpose of this study was to investigate TAF phenotype and functions comparative to bone marrow-derived mesencymal stem cells (MSCs) and to evaluate the role of these cells in tumorigenesis. TAFs were isolated using enzymatic digestion from surgical removed breast tumor pieces and were cultured for several passages in standard cultivation conditions, similar to MSCs. Cellular analysis included transmission electron microscopy (TEM), immunophenotyping (HLA-DR, CD29, CD44, CD73, CD90, CD106, and CD117), cytoskeleton and matrix proteins expression based on immunohistochemistry, and tri-lineage differentiation potential towards adipocytes, osteoblasts, and chondrocytes. ELISA assay was used for determining cells secretory profile, and we found that TAFs secrete significantly increased amounts of VEGF, TGF-β1, IL-4, IL-10, and TNF-α. Tumor cell lines SK-BR3, MDA-MB231 and MDA-MB468 were used for flowchamber adherence studies, under progressively increasing shear stress from 0.35 to 15 dyne/cm2, on MSCs or TAFs cellular substrate. The results showed that tumor cells have increased adherence to TAFs, for all values of shear stress, mainly because VEGF and IL-4 secretion, so that we may conclude that even though MSCs and TAFs share functional and structural characteristics, TAFs represent a more specialized cellular type, able to provide support for tumor growth and development, and can be a target for further anti-tumor therapies.
Acknowledgements
PN II IDEI 318/2011
Keywords:
MSCs,
TAFs,
tumorigenesis,
immunemodulation,
Angiogenesis
Conference:
15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.
Presentation Type:
Abstract
Topic:
Translational immunology and immune intervention
Citation:
Toma
M,
Lazarovicz
R,
Gavriliuc
O,
Bojin
FM,
Tatu
C,
Gruia
A,
Tatu
C,
Tanasie
G,
Panaitescu
C and
Paunescu
V
(2013). Tumor associated fibroblasts are endowed with increased ability to support tumor development.
Front. Immunol.
Conference Abstract:
15th International Congress of Immunology (ICI).
doi: 10.3389/conf.fimmu.2013.02.00446
Copyright:
The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers.
They are made available through the Frontiers publishing platform as a service to conference organizers and presenters.
The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated.
Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed.
For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions.
Received:
15 Apr 2013;
Published Online:
22 Aug 2013.
*
Correspondence:
Dr. Marius Toma, University of Medicine and Pharmacy Victor Babes Timisoara, Functional Sciences, Timisoara, 300041, Romania, tomamarius.forever@gmail.com