Migration of human mesenchymal stem cells towards inflammatory lesions after transfection with CD29 specific siRNA
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1
Victor Babes University of Medicine and Pharmacy Timisoara, Romania
Background and aims: Mesenchymal stem cells (MSCs) are a population of non-hematopoietic stem cells with differentiation potential, which have the capacity to migrate towards inflammatory lesions. Several adhesion molecules, chemokines, and integrins are involved in the MSC response to inflammation. The aim of this study was to investigate the specific role of integrin beta1 (CD29) in MSC migration towards inflammatory skin lesions, in order to increase the MSC efficiency in wound healing.
Method: A GFP-expressing human MSC line (hMSCs) was purchased. The cell expression of CD29 was inhibited specifically by transfection with siRNA duplexes. An inflammatory skin lesion was produced in CD1 Nu/Nu mice, and the transfected hMSCs were injected subcutaneously. hMSC migration in vivo was evaluated macroscopically by a fluorescence imaging system and microscopically by immunofluorescence for CD29, vimentin, cytokeratin and Ki67.
Results: The intracellular and surface expression of CD29 was abundant, as shown by immunohistochemistry and flow cytometry (74.22±2.3% positive cells), respectively. CD29 was successfully inhibited, its expression being reduced by 76% in transfected hMSCs (18.02% positive cells). Transfected hMSCs also exhibited decreased expression of CD90 and CD105. When compared with control hMSCs, transfected hMSCs migrated more slowly to the skin inflammatory lesion site, but their proliferation rate was higher, as shown by their expression of Ki67. However, there were no significant differences between overall healing times.
Conclusion: CD29 inhibition has downstream effects by affecting the expression of MSC specific markers and MSC proliferation. It may offer a novel approach to the treatment of inflammatory skin lesions.
Keywords:
Mesenchymal Stem Cells,
CD29,
Inflammation Mediators,
siRNA,
Migration
Conference:
15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.
Presentation Type:
Abstract
Topic:
Immune receptors and signaling
Citation:
Marusciac
L,
Panaitescu
C,
Bojin
F and
Paunescu
V
(2013). Migration of human mesenchymal stem cells towards inflammatory lesions after transfection with CD29 specific siRNA.
Front. Immunol.
Conference Abstract:
15th International Congress of Immunology (ICI).
doi: 10.3389/conf.fimmu.2013.02.00422
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Received:
13 Apr 2013;
Published Online:
22 Aug 2013.
*
Correspondence:
Dr. Laura Marusciac, Victor Babes University of Medicine and Pharmacy Timisoara, Timisoara, Romania, laura.marusciac@gmail.com