Predominant Th1 cytokines in seronegative, aviremic children who exhibit HCV-specific cell-mediated immune response
Maha
S.
Abdelmegied1*,
Mohamed
Hashem1, 2,
Sayed
F.
Abdelwahab1, 3,
Ahmed
B.
Barakat4,
Hanaa
El-Karaksy5,
Gehan
Galal1,
Nabiel
Mikhail6,
Samer
S.
El-Kamary2,
G.
T.
Strickland2 and
Mohamed
T.
Shata7
-
1
The Egyptian holding company for biological products and vaccines (Egyblood/Vacsera), Egypt
-
2
University of Maryland School of Medicine, Department of Epidemiology and Public Health, United States
-
3
Faculty of Medicine, Minia University, Department of Microbiology and Immunology, Egypt
-
4
Faculty of Science, Ain Shams University, Department of Microbiology, Egypt
-
5
Faculty of Medicine, Cairo University, Department of Pediatrics, Egypt
-
6
South Egypt Cancer Institute. Assiut University, Cancer Epidemiology Department, Egypt
-
7
University of Cincinnati, Division of Digestive Diseases, Department of Internal Medicine, United States
Background: A predominant T helper 1 (Th1) response in hepatitis C virus (HCV)-infected adults is associated with viral clearance. We investigated the role of both Th1 and Th2 cytokines in seronegative, aviremic children at high risk of HCV exposure.
Methods: Peripheral blood mononuclear cells (PBMC) from nine seronegative aviremic children at high risk of HCV exposure, and from two seropositive HCV-viremic children were stimulated in vitro with 3 recombinant HCV protein antigens and cell-mediated immune responses (CMI) were measured using IFN-γ ELISPOT assays. Additionally, supernatants from these cell cultures were tested for Th1 and Th2 cytokines.
Results: Significantly higher IFN- γ HCV-specific responses were detected in five of the nine seronegative, aviremic children at high risk of HCV exposure. No CMI responses were elicited in the viremic siblings. Additionally, TNF-α levels in response to NS3/NS4 HCV antigens is significantly higher (p= 0.013) in the supernatant of the five CMI responders compared to the viremic siblings.
Conclusion:
The presence of Th1 HCV-specific TNF- α and IFN- γ cytokines in seronegative, aviremic CMI responders and their absence in their HCV-infected siblings suggests that children at high risk of HCV exposure were most likely to have transien infection, and their Th1 immune responses were protective
Keywords:
Hepatitis C virus,
Children,
Cell Mediated Immune Response,
Cytokines,
TNF-a
Conference:
15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.
Presentation Type:
Abstract
Topic:
Host-pathogen interactions
Citation:
Abdelmegied
MS,
Hashem
M,
Abdelwahab
SF,
Barakat
AB,
El-Karaksy
H,
Galal
G,
Mikhail
N,
El-Kamary
SS,
Strickland
GT and
Shata
MT
(2013). Predominant Th1 cytokines in seronegative, aviremic children who exhibit HCV-specific cell-mediated immune response.
Front. Immunol.
Conference Abstract:
15th International Congress of Immunology (ICI).
doi: 10.3389/conf.fimmu.2013.02.00378
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Received:
22 Mar 2013;
Published Online:
22 Aug 2013.
*
Correspondence:
Dr. Maha S Abdelmegied, The Egyptian holding company for biological products and vaccines (Egyblood/Vacsera), Giza, Egypt, maha_sobhy@hotmail.com