Basophil-derived mouse mast cell protease 11 is involved in the development of IgE-mediated chronic allergic inflammation
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1
Tokyo Medical and Dental University, Japan
Basophils are the least common granulocytes, and had long been considered as minor relatives of mast cells. However, recent studies have illustrated non-redundant roles for basophils in various immune responses, including allergic reactions and protective immunity against parasites. We previously demonstrated that basophils but not mast cells play an important role in the development of IgE-mediated, delayed-onset allergic inflammation in the skin, designated IgE-CAI. However, it remains elusive how basophils trigger the skin inflammation. In this study, we focused on mast cell protease 11 (mMCP-11) that shows a tryptase activity, and is expressed by basophils rather than mast cells. In order to elucidate the role for mMCP-11 in IgE-CAI, we generated engineered mice deficient for mMCP-11, in that the number of basophils was comparable to that in wild-type mice. Their basophils expressed normal levels of other proteases including mMCP-8 and surface FcεRI, and normally degranulated upon stimulation with IgE plus antigens, as judged by β-hexosaminidase release assay. Of note, skin swelling elicited by the IgE-CAI reaction was reduced in mMCP-11-deficient mice to half of that in wild-type mice. Moreover, the number of proinflammatory cells accumulating skin lesions, including eosinophils and neutrophils, was significantly reduced in mMCP-11-deficient mice. Thus, mMCP-11 released by basophils is a key effector molecule in the development of IgE-CAI.
Keywords:
basophil,
mMCP-11,
allergic inflammation,
IgE,
Tryptases
Conference:
15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.
Presentation Type:
Abstract
Topic:
Adaptive Immunity
Citation:
DEKI
H,
KAWANO
Y,
YOSHIKAWA
S,
EGAWA
M,
IKI
M and
KARASUYAMA
H
(2013). Basophil-derived mouse mast cell protease 11 is involved in the development of IgE-mediated chronic allergic inflammation.
Front. Immunol.
Conference Abstract:
15th International Congress of Immunology (ICI).
doi: 10.3389/conf.fimmu.2013.02.00356
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Received:
19 Mar 2013;
Published Online:
22 Aug 2013.
*
Correspondence:
Prof. Hajime KARASUYAMA, Tokyo Medical and Dental University, Tokyo, Japan, karasuyama.mbch@tmd.ac.jp