Expression of TCR trangenic beta-chain leads to contraction of T lymphocytes pools with surface phenotypes of effector and central memory cells.
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1
Federal State Budgetary Institution”N.N.Blokhin Cancer Research Center”, Russian Academy of Medical Sciences, Russia
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2
Institute of Gene Biology, Russian Academy of Sciences, Russia
Naive T lymphocytes in mice can be distinguished from antigen-experienced T cells by different coexpression of activation markers, such as CD44 and CD62L. These markers determine different traffic of T lymphocytes in the organism, but hardly reproduce real antigenic experience of a T lymphocyte. Mechanisms maintaining homeostasis of T lymphocytes with different activation phenotypes remain largely unknown. To investigate impact of transgenic chains of T cell receptor (TCR) on formation of T lymphocytes, their peripheral survival and activation surface phenotypes, we have generated transgenic mouse strain expressing transgenic beta-chain of TCR 1D1 (belonging to the Vb6 family) on the genetic background B10.D2(R101). Intrathymic development of T cells in these transgenic mice is not impaired. 70-80% of peripheral T cells express transgenic beta-chain and only 20-30% of peripheral T cells express endogenous beta-chains. The ratio of peripheral CD4+CD8- and CD4-CD8+ T lymphocytes stays unchanged in transgenic animals. However the percentage of T lymphocytes with the “naive” phenotype CD44-CD62L+ is significantly increased and the levels of effector memory CD44+CD62L- and central memory CD44+CD62L+ T lymphocytes are markedly decreased in both subpopulations. On the contrary, T lymphocytes expressing endogenous beta-chains have the surface phenotype of activated T cells CD44+. Thus, we have shown for the first time that pools size of T lymphocytes with different activation phenotypes depends on the structure of T cell receptors.
Keywords:
T cell receptor,
Transgenic Animals,
lymphocyte,
effector,
memory cell
Conference:
15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.
Presentation Type:
Abstract
Topic:
Immune receptors and signaling
Citation:
Silaeva
Y,
Kalinina
A,
Vagida
M,
Khromykh
L,
Deikin
A,
Ermolkevich
T,
Sadchikova
E,
Goldman
I and
Kazansky
D
(2013). Expression of TCR trangenic beta-chain leads to contraction of T lymphocytes pools with surface phenotypes of effector and central memory cells..
Front. Immunol.
Conference Abstract:
15th International Congress of Immunology (ICI).
doi: 10.3389/conf.fimmu.2013.02.00339
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Received:
15 Mar 2013;
Published Online:
22 Aug 2013.
*
Correspondence:
Ms. Yulia Silaeva, Federal State Budgetary Institution”N.N.Blokhin Cancer Research Center”, Russian Academy of Medical Sciences, Moscow, Russia, yulya.silaeva@gmail.com