Modulation of NKG2D and NKG2A ligands expression during monocyte differentiation
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1
Sapienza University of Rome, Dept Biology and Biotechnology "C. Darwin", Italy
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2
Sapienza University of Rome, Dept of Experimental Medicine, Italy
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3
Sapienza University of Rome, Dept of Molecular Medicine, Italy
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4
Istituto Pasteur - Fondazione Cenci Bolognetti, Sapienza University of Rome, Italy
Natural killer cells and macrophages are the major effectors of the innate immune system. Crosstalk between these two populations is still poorly understood although it is likely to have strong implications in the development of both innate and adaptive immune response. NK cells express a plethora of activating and inhibitory receptors which bind different ligands on target cells. HLA-E, a non classical MHC class I molecule, binds the inhibitory CD94/NKG2A receptor thus protecting cells from the NK killing. The expression and function of HLA-E require peptides derived from the leader sequence of HLA class I molecules. However, HLA-E molecules can also bind self or pathogen-derived peptides, whose effect on the NK response is still controversial. We show here that the expression of the HLA-E molecules is strongly up-regulated during differentiation of monocytes to macrophages along with other molecules involved in HLA class I antigen processing and presentation such as ERAP1 and ERp57 whereas, the expression of classical HLA class I molecules remains unaffected. These findings strengthen the hypothesis that HLA-E exploits the same machinery as the classical HLA-class I molecules to present self proteins. Ligands (MIC-A/B, ULBPs) for the activating NKG2D receptors are also modulated during monocyte differentiation. The influence of this strong modulation on the crosstalk between NK cells and macrophages is under investigation
Keywords:
HLA-E,
monocytes/macrophages,
NK,
differentiation,
NKG2A,
NKG2D ligands
Conference:
15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.
Presentation Type:
Abstract
Topic:
Innate immunity
Citation:
Camilli
G,
Paladini
F,
Cassotta
A,
La Scaleia
R,
Battella
S,
Fiorillo
M,
Palmieri
G,
Santoni
A and
Sorrentino
R
(2013). Modulation of NKG2D and NKG2A ligands expression during monocyte differentiation.
Front. Immunol.
Conference Abstract:
15th International Congress of Immunology (ICI).
doi: 10.3389/conf.fimmu.2013.02.00330
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Received:
22 Mar 2013;
Published Online:
22 Aug 2013.
*
Correspondence:
Dr. Giorgio Camilli, Sapienza University of Rome, Dept Biology and Biotechnology "C. Darwin", Rome, Italy, giorgio.camilli@uniroma1.it