T cell activation markers and inflammatory cytokine profile in patients with diabetic nephropathy undergoing hemodialysis
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1
University of Beira Interior, Portugal
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2
CICS-UBI, Health Sciences Research Centre, University of Beira Interior, Covilhã, Portugal, Portugal
Diabetic nephropathy (DN) is a common complication disease in diabetic patients, and most of them need to be integrated into renal replacement therapy such as hemodialysis (HD). Our objective was to access the expression the activation markers on T cells, as well as the synthesis of inflammatory cytokines, before and after HD.
The study was approved by the local ethics committee and involved 17 patients with DN under HD treatment which signed an informed consent. Blood samples before and after one HD session were collected, in order to analyze the expression of CD25, CD69 and CD71 in T cells. We also analyzed the synthesis of IL-12p70, IL-8, IL-10, IL-1β, TNF-α and IL-6 in serum samples by Cytometric Bead Array.
After the HD session, there was an increase in CD4/CD8 ratio due to significant alterations in both subsets. The relative percentage of CD25+ T cells and CD8+CD25+ increased significantly after the HD session, while the relative percentage of CD69 T cells decreased. There was a significant decrease of the CD25 Mean Fluorescence Intensity values for CD4+ T, as well as in the case of CD71 in T cell sub-populations, after the HD session. Regarding cytokine synthesis in serum samples, we found a significant increase in IL-10 and IL-6 and a decrease in IL-8 after HD session.
This study showed that a HD session in DN patients affects the T cell activation status, and differentially modulates the production of inflammatory cytokines.
Funding: CENTRO-07-ST24-FEDER-002015
Acknowledgements
Funding: CENTRO-07-ST24-FEDER-002015
Keywords:
diabetic nephropathy,
hemodialysis,
T cells,
Cytokines,
Activation markers
Conference:
15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.
Presentation Type:
Abstract
Topic:
Adaptive Immunity
Citation:
Almeida
A,
Costa
N,
Lourenço
O and
FONSECA
A
(2013). T cell activation markers and inflammatory cytokine profile in patients with diabetic nephropathy undergoing hemodialysis.
Front. Immunol.
Conference Abstract:
15th International Congress of Immunology (ICI).
doi: 10.3389/conf.fimmu.2013.02.00307
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Received:
21 Mar 2013;
Published Online:
22 Aug 2013.
*
Correspondence:
Prof. ANA MAFALDA FONSECA, University of Beira Interior, Covilhã, Portugal, mfonseca@fcsaude.ubi.pt