Event Abstract

Immunotherapeutic effects of recombinant adenovirus encoding granulocyte–macrophage colony-stimulating factor in experimental pulmonary tuberculosis

  • 1 National Institute of Medical Sciences and Nutrition Salvador Zubirán, Experimental Pathology, Mexico
  • 2 National School of Biological Sciences, National Polytechnic Institute, Immunology, Mexico
  • 3 McMaster University, Pathology & Molecular Medicine, Canada

BALB/c mice with pulmonary tuberculosis (TB) develop a T helper cell type 1 that temporarily controls bacterial growth. Bacterial proliferation increases, accompanied by decreasing expression of interferon (IFN)-g, tumour necrosis factor (TNF)-a and inducible nitric oxide synthase (iNOS). Activation of dendritic cells (DCs) is delayed. Intratracheal administration of only one dose of recombinant adenoviruses encoding granulocyte–macrophage colony-stimulating factor (AdGM-CSF) 1 day before Mycobacterium tuberculosis (Mtb) infection produced a significant decrease of pulmonary bacterial loads, higher activated DCs and increased expression of TNF-a, IFN-g and iNOS. When AdGM-CSF was given in female mice B6D2F1 (C57BL/6J X DBA/2J) infected with a low Mtb dose to induce chronic infection similar to latent infection and corticosterone was used to induce reactivation, a very low bacilli burden in lungs was detected, and the same effect was observed in healthy mice co-housed with mice infected with mild and highly virulent bacteria in a model of transmissibility. Thus, GM-CSF is a significant cytokine in the immune protection against Mtb and gene therapy with AdGM-CSF increased protective immunity when administered in a single dose 1 day before Mtb infection in a model of progressive disease, and when used to prevent reactivation of latent infection or transmission.

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Keywords: Latent Tuberculosis, murine model, Immunotherapy, transmission, Granulocyte-Macrophage Colony-Stimulating Factor, Gene Therapy

Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.

Presentation Type: Abstract

Topic: Translational immunology and immune intervention

Citation: Francisco-Cruz A, Hernandez-Pando R, Mata-Espinosa D and Xing Z (2013). Immunotherapeutic effects of recombinant adenovirus encoding granulocyte–macrophage colony-stimulating factor in experimental pulmonary tuberculosis. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00283

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Received: 13 Mar 2013; Published Online: 22 Aug 2013.

* Correspondence: Dr. Alejandro Francisco-Cruz, National Institute of Medical Sciences and Nutrition Salvador Zubirán, Experimental Pathology, Mexico, Mexico City, 14000, Mexico, inhoutes@yahoo.com