Immune mechanisms in periodontitis – effect of IL-4 gene polymorphisms on cytokine production
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1
Institute of Clinical and Experimental Dental Medicine , GUH and First Faculty of Medicine Charles University, Czechia
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2
Faculty of Medicine Masaryk University, Department of Physiology, Czechia
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3
Clinic of Stomatology, Faculty of Medicine MU and St Anne´s Hospital, Czechia
Introduction: Periodontitis is a chronic inflammatory disease of the teeth-supporting tissues in which genetic predisposition, dental plaque bacteria and immune mechanisms play important roles. The aim of this study was to assess the influence of IL-4 gene polymorphisms on cytokine production
Methods: Cytokine production (IL-1α, IL-1β, IL-4, IL-5, IL-6, IL-8 , IL-10, TNF–α, INF–γ) was studied after in vitro stimulation of isolated peripheral blood mononuclear cells (PBMC) by mitogens and dental plaque bacteria (Aggregatibacter actinomycetemcomitans, Escherichia coli, Tannerrella forsythensis, Porphyromonas gingivalis and Heat Shock Protein 60). Cytokine production was detected by multiplex cytokine analysis system Luminex. The results were correlated with IL-4 genotypes in patients with chronic periodontitis (CP) and healthy controls.
Results: We found that mononuclear cells isolated from peripheral blood of CP patients with investigated IL-4 polymorphisms (-589C/T, -33C/T, VNTR in intron 3) significantly changed production of IFN-, IL-10, IL-1 and IL-1, TNF-, IL-10, MCP-1, IL-6 after stimulation by all selected bacteria and HSP (p0.05).
Conclusion: In patients with CP, IL-4 polymorphisms may influence the function of mononuclear cells to produce not only IL-4 but also other cytokines. The results of this study support Seymour’s hypothesis (1999) about the role of TH2 produced cytokines such as IL-4 in genetic predisposition to chronic periodontitis.
Acknowledgements
The study was supported by PRVOUK-P28/LF1/6 (Ministry of Education, Youth and Sports, Czech Republic) and by grant IGA NT11405-6.
Keywords:
Periodontitis,
immune mechanisms,
production of cytokines,
IL-4,
IL-4 gene polymorphismus
Conference:
15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.
Presentation Type:
Abstract
Topic:
Immune-mediated disease pathogenesis
Citation:
Bartova
J,
Borilova Linhartova
P,
Podzimek
S,
Svobodová
K,
Janatova
T,
Fassmann
A,
Duskova
J and
Izakovicova-Holla
L
(2013). Immune mechanisms in periodontitis – effect of IL-4 gene polymorphisms on cytokine production.
Front. Immunol.
Conference Abstract:
15th International Congress of Immunology (ICI).
doi: 10.3389/conf.fimmu.2013.02.00278
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Received:
13 Mar 2013;
Published Online:
22 Aug 2013.
*
Correspondence:
Dr. Jirina Bartova, Institute of Clinical and Experimental Dental Medicine , GUH and First Faculty of Medicine Charles University, Prague, Czechia, jirina.bartova@post.cz